Lab Practical II Flashcards
Enzyme that breaks down hydrogen peroxide
Catalase
Source of Catalase in experiments
Sheep’s blood
Products of catalase use
Hdyrogen Peroxide —-> Water + Oxygen
Measured product for the experiment
Oxygen
Optimum Enzyme pH
8
Why is catalase enzyme less active at very low temperatures or very high temperatures?
At high temperature, the enzyme (protein) denatures. At low temperatures, the number of successful collisions between the enzyme and substrate is reduced because their molecular movement decreases.
Why are enzymes less active for very high or low pH?
High or low pH can cause enzymes to denature or cause the enzyme to change shape. These things prevent the enzyme from working properly.
What molecule is the substrate in exercise 6?
Hydrogen Peroxide
What is the control in this experiment?
Water
Is the control a positive or negative control?
Negative. It should not produce any kind of change.
As substrate concentration increases, the reaction rate __________.
Increases
As substrate concentration decreases the reaction rate ___________.
decreases
As enzyme concentration decreases, reaction rate __________
decreases
Name and chemical formula of the inhibitor.
CuSO4 (Copper sulfate)
Expected result of using CuSO4 (an inhibitor)
To reduce the reaction rate.
