Lab Practical II Flashcards
Enzyme that breaks down hydrogen peroxide
Catalase
Source of Catalase in experiments
Sheep’s blood
Products of catalase use
Hdyrogen Peroxide —-> Water + Oxygen
Measured product for the experiment
Oxygen
Optimum Enzyme pH
8
Why is catalase enzyme less active at very low temperatures or very high temperatures?
At high temperature, the enzyme (protein) denatures. At low temperatures, the number of successful collisions between the enzyme and substrate is reduced because their molecular movement decreases.
Why are enzymes less active for very high or low pH?
High or low pH can cause enzymes to denature or cause the enzyme to change shape. These things prevent the enzyme from working properly.
What molecule is the substrate in exercise 6?
Hydrogen Peroxide
What is the control in this experiment?
Water
Is the control a positive or negative control?
Negative. It should not produce any kind of change.
As substrate concentration increases, the reaction rate __________.
Increases
As substrate concentration decreases the reaction rate ___________.
decreases
As enzyme concentration decreases, reaction rate __________
decreases
Name and chemical formula of the inhibitor.
CuSO4 (Copper sulfate)
Expected result of using CuSO4 (an inhibitor)
To reduce the reaction rate.
Balanced chemical equation of the catalysis of Hydrogen Peroxide using Catalase
2H2O2 —-> 2H2O + O2
Equation for photosynthesis
H2O + CO2 + Energy = C6H12O6 + O2
Equation for Cellular Respiration
C6H12O6 + O2 = CO2 + H2O + Energy (ATP)
What Plant genus was used in this expiriment?
Elodea
What is the purpose of the beaker of water between the light source and the leaves?
It keeps the leaves from becoming too hot. In other words, the beaker of water is a heat shield (or heat sink).
The variegated leaf used in part B of the experiment is
Coleus
Pigments of the Coleus Leaf
Chlorophyll (green)
anthocyanin (red)
combined they are brick red
Pigment which is alcohol soluble
Chlorophyll
Pigment which is water soluble
anthocyanin
What chemical was used as an indirect indicator that photosynthesis had taken place on a particular area of the leaf?
IKI
What color is seen when IKI reacts with starch?
A Blue Black Color
Where can starch be used as a test for photosynthesis on a leaf?
In the green areas where chlorophyll can be found.
What factors affect the distance that pigment molecules travel on chromotography paper?
The solubility of the pigments in the chromotography solvent, and the molecular weights of the pigments.
