Lab Practical 2 Flashcards
What color does the wavelength around 400 correspond to?
Purple
What color does the wavelength around 600 correspond to?
Blue
What color does the center of the wavelength spectrum best correspond to?
Green
What color does the wavelength around 700 correspond to?
Red
What is the independent variable in the chlorophyll extraction lab?
Wavelength
What is the dependent variable in the chlorophyll extraction lab?
Absorbance
Which pigment is more soluble in ethanol?
Carotene
Which pigment is more soluble in petroleum ether?
Chlorophyll A
Which pigment is more soluble in methanol?
Chlorophyll B
Pigments do not absorb what kind of light well?
Green, it reflects it and that is why chlorophyll appears green
What does water contain that plants need?
Low energy electrons
What do plants need to do to low energy electrons?
Extract and energize
What is DPIP?
A lipid soluble dye
What color does DPIP turn when oxidized?
Blue
What color does DPIP turn when reduced?
Clear
What is the independent variable in the oxidation of water lab?
mL of chloroplasts
What is the dependent variable in the oxidation of water lab?
Rate of reaction
What is the relationship between the variables in the oxidation of water lab?
Direct linear
What does KCl do in the oxidation of water lab?
Simulates a natural state for chloroplasts to keep them oxidizing water
What does Bisulfite do in the oxidation of water lab?
Causes DPIP to lose color in the blank
What did the buffer do in the oxidation of water lab?
Stabilize pH
What is the independent variable in the oxygen production lab?
PFR (photon fluence rate) which was measured by changing the distance from the light source
What is the dependent variable in the oxygen production lab?
Rate of O2 production
What is the relationship between the variables in the oxygen production lab?
Direct linear
