Lab Practical 1 Flashcards
Terms to expect on the first Bio 110 lab practical
What prokaryotes live in strange and hostile habitats?
Archaea
What prokaryotes have peptidoglycan cell walls?
Eubacteria
What is the defining feature of prokaryotes as a group?
No membrane bound nucleus
What eubacteria is spherical?
Coccus/cocci
What eubacteria is rod-shaped?
Bacillus/bacilli
What eubacteria is helical?
Spirilla
What kind bacteria feed on other things?
Heterotrophic
What kind of bacteria are self feeders?
Autotrophic
Requires oxygen
Aerobic
Does not require oxygen
Anaerobic
What major group of bacteria is photosynthetic?
Cyanobacteria
Non-photosynthetic autotrophs are called what?
Chemosynthetic autotrophs
What is the defining characteristic of eukaryotes?
Membrane bound nucleus
What eukaryotes cannot be identified as fungi, plants, or animals?
Protists
What eukaryotes contain cavities called alveoli just beneath their plasma membranes?
Alveolata
What alveolata is an apicomplexan found in blood?
Plasmodium
What is an apical complex?
A cytoskeleton apparatus
What alveolata is a ciliate (moves by cilia)?
Paramecium
What alveolata is a dinoflagellate?
Peridinium
What is a dinoflagellate?
A unicellular photosynthetic autotroph
What eukaryotes have fine hairs on their flagella?
Stramenopiles
What multicellular stramenopile has rounded, bulb-like ends?
Fucus
What multicellular stramenopile appears thin and whispy?
Sargassum
What unicellular stramenopile has a silica shell?
Diatoms
What group of protists have slender pseudopodia?
Rhizaria
What rhizaria has a silica shell with perforated holes?
Radiolaria
What group of eukaryotes have modified mitochondria?
Excavata
What type of excavata use flagella to move?
Euglenozoa
What euglenoza excavata contains kinetoplast?
Trypanosoma
What type of excavata has two nuclei?
Diplomonads
What type of diplomonad excavata is a water-borne parasite?
Giardia
What group of eukaryotes have blunt pseudopodia?
Ameobozoans
What type of ameobozoans move by way of pseudopodia?
Amoeba
What is movement by pseudopodia?
Flexible extensions that reach out, attach, and then pull the amoeba along
What type of amoeba was observed in the lab?
Amoeba proteus
What eukaryotes have chitin cell walls?
Fungi
What distinguishes fungi groups from each other?
The type of sexual reproduction
What group of fungi reproduce sexually by the hyphae?
Zygomycota
What type of zygomycota was observed in the lab?
Rhizopus
What type of fungi reproduce by structures called asci?
Ascomycota
What type of ascomycota that is used for baking and brewing was observed in the lab?
Saccharomyces
What type of fungi reproduces sexually via basidia?
Basidiomycota
What type of basidiomycota was observed in the lab?
Club mushroom
What are proteins?
Polymers of amino acids that are linked by peptide bonds
Each protein has at least…
One carboxyl group and one amino group
Carboxyl groups typically have what kind of charge?
Negative
Amino groups typically have what kind of charge?
Positive
The ____ groups can also determine the charge of amino acids.
R
What category of R groups matters for electrophoresis?
Ionic
What 5 amino acids have ionic R groups?
Aspartic acid, glutamic acid, lysine, arginine, and histidine
The carboxyl group has what charge at low environmental pH?
Net zero
The carboxyl group has what charge at high environmental pH?
Negative
What amino acids have a carboxyl group that can be negatively charged when the environment is at a high pH?
Aspartic acid and glutamic acid
What amino acids have one or amino or imino groups that may carry a positive charge?
Lysine, arginine, and histidine
What happens to an electrically neutral amino group at an low environmental pH?
Net charge of plus one
When hydroxyl ions from a high environmental pH react with a positively charged amino group, what is the charge on the amino group?
Net zero charge
What is the pH point that a particular protein will have no net charge?
Isoelectric point
What is the independent variable of the electrophoresis lab?
pH
What is the dependent variable of the electrophoresis lab?
The net electrical charge on each protein
What is the cloudy mixture in the permeability lab called?
Turbid
What happens when the cells are over saturated and burst?
The cells lyse
What is the value used to determine the time required to reach one-half the maximum value indicated on the plot?
H50 (hemolysis 50)
H50 measures what?
Time to lysis
What were the three independent variables of the permeability lab?
Size of the solute molecule, the net charge of the solute particle, and the lipid solubility of the solute molecule
What was the dependent variable in the permeability lab?
Cell membrane permeability
What kind of molecules are not well controlled by the cell membrane?
Lipid soluble molecules
What is the ratio used to determine how lipid soluble a molecule will be?
CH(n)/OH
What kind of molecules can not easily pass through membranes?
Water soluble molecule
What is synapomorphy?
The new, defining characteristic/feature that a group shares