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Cchm Lec > LAB PPT > Flashcards

LAB PPT Flashcards

1
Q

Most common Resistant Borosilicate:

A

–Beakers
–Flasks
–Pipettes

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2
Q

High thermal resistant glass Usual brand that may encounter was _______ and __________

A

Kimax and pyrex

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3
Q

This has high resistance to thermal shock and chemical attack

A

Borosilicate/ high thermal resistant glass

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4
Q

Can be heated and autoclave

A

High thermal resistant glass/ borosilicate

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5
Q

Made by removing all elements from borosilicate glass but 6 times stronger than borosilicate glass

A

High Silica Glass (COREX)

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6
Q

Has good optical qualities, temperature capabilities and
is radiation-resistant

A

High Silica Glass (COREX)

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7
Q

Used for high precision analytical work and for optical
reflectors and mirrors

A

High Silica Glass (COREX)

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8
Q

Not used for the type of glassware generally used in the
laboratory

A

High Silica Glass (COREX)

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9
Q

Partially used for strong alkaline solutions

A

High Alkali-Resistant Glass

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10
Q

Often referred to as “soft glass” as its thermal
resistance is much less than of borosilicate glass

A

High Alkali-Resistant Glass

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11
Q

digestion with strong
alkali is made

A

High Alkali-Resistant Glass

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12
Q

poor in heat resistance therefore it is
not autoclavable

A

High Alkali-Resistant Glass

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13
Q

Has materials that usually impart red color to the glass that reduce the amount of light passing
through substance inside the glassware

A

Low actinic glass

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14
Q

Low actinic glass Provides protection to reagents highly sensitive
to light ranging from __________ to __________ angstrom

A

3,000 to 5,000 angstrom

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15
Q

Low actinic glass Used for substances that are particularly
sensitive to light such as __________ or _________ (other substance)

A

bilirubin or Vitamin A ( beta carotene, vitamin c, vitamin b1, porphyrin, solate)

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16
Q

Used for the manufacture of
weighing bottles because it
develops less static surface
changes

A

Standard Flint Glass or Soda
Lime Glass

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17
Q

Standard Flint Glass or Soda
Lime Glass Composed of a mixture of the
___________, __________ and ________

A

oxides, Calcium and silicon

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18
Q

Special Glasses: Used in light fitters, lamp bulbs and lightning lenses (fluorescent light)

A

Colored and Opal Glasses

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19
Q

Special glass: Have thin metallic oxide permanently fine bonded to the surface of the glass (infrared light)

A

Coated Glasses

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20
Q

Special glass: Have electronic applications as heat shield to protect against
infrared light

A

Coated Glasses

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21
Q

Special glass: Mostly soda lime glass lead and borosilicate of high optical purity (used spectrophotometer)

A

Optical Glasses

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22
Q

Special glass: Used in making prisms, lenses and optical

A

Optical Glasses

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23
Q

Special glass: Useful in hot plates, table tops and heat exchanges

A

Glass Ceramics (Pyroceram)

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24
Q

Special glass: Have high thermal resistance, chemical stability and corrosion resistance like borosilicate glasses

A

Glass Ceramics (Pyroceram)

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25
Q

Special glass: Made of soda-lime and lead

A

Radiation - absorbing glass

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26
Q

Special glass: Useful in preventing transmission of huge energy radiation as gamma
rays and X-rays

A

Radiation - absorbing glass

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27
Q

Beginning to replace glassware in the laboratory setting

A

Plastic wares

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28
Q

Its unique high resistance to corrosion and breakage as
well as its varying flexibility, had made it most appealing

A

Plastic wares

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29
Q

Its relatively expensive, allowing for most items to be
completely disposable after each use

A

Plastic wares

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30
Q

Unique group of resins with relatively inert properties

A

Polyolefins

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31
Q

Unaffected by acids, alkalis, salt solutions and aqueous solutions and can be autoclave

A

Polyolefins

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32
Q

Two types of polyolefins:

A

Polypropylene

Polyethylene

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33
Q

Type of polyolefins: Can withstand higher temperatures

A

Polypropylene

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34
Q

Type of polyolefins: More vulnerable to attack by oxidizing agents

A

Polypropylene

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35
Q

Type of polyolefins: Both polypropylene are used primarily to fabricate bottles, beakers,
jars jugs, funnels pipette jars, pipette baskets, tanks, etc.

A

Polyethylene

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36
Q

Twice as strong as polypropylene and may be used at temperatures ranging 100C to 160C

A

Polycarbonate resin

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37
Q

Unsuitable for use with bases such as amines, ammonia, alkalis, and
oxidizing agents

A

Polycarbonate resin

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38
Q

Used extensively in centrifuge tubes and graduated cylinder

A

Polycarbonate resin

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39
Q

Non-toxic, clear plastic of modified plasticized polyvinyl chloride

A

Tygon

40
Q

Flexible at 30C, brittle at 45C and resists dry heat to 95C

A

Tygon

41
Q

Used for the manufacture of tubing (i.e, tubing used in
Automated Analyzers)

A

Tygon

42
Q

Pure translucent white and inert to corrosive reagents boiling agua regia, nitric and sulphuric acids, boiling hydrocarbons, ketones, esters and alcohols

A

Telfon-fluorocarbon resin

43
Q

Used for self-lubricating stopcocks, stirring bars, bottle cap liners
and tubing because of its anti-adhesive properties

A

Telfon fluorocarbon resin

44
Q

Can resist extreme temperatures ranging from 270C to 255C

A

Telfon flurocarbon resin

45
Q

Mercury does not wet glass and pipette
calibrated this way will contain but not
deliver the started volume

A

TC (To Contain) Pipette

46
Q

Rate of delivery must never be hastened by
blowing

A

TD (To Deliver) Pipette

47
Q

An etched ring is seen near the
mouthpiece

A

“To Blow-Out” Pipette

48
Q

The user allows the contents of the pipette
to drain by gravity

A

Self Draining pipette

49
Q

Has the greatest degree of accuracy and
precision and self draining

A

Volumetric / transfer Pipette

50
Q

Used in measuring viscous fluids –Measures smaller volume and blow out pipette

A

Ostwald folin pipette

51
Q

Used to deliver an amount of liquid
contained between two calibration on
marks

A

Graduated/measuring Pipette

52
Q

The rate of fall of liquid is much too fast. It has Has an etched band on the suction and blow out pipette

A

Serologic Pipette

53
Q

The rate of fall of liquid is much too fast. It has Has an etched band on the suction and blow out pipette

A

Serologic Pipette

54
Q

Calibration lies between two marks without graduations to the top and Self-draining pipette

A

Mohr Pipette

55
Q

To contain” pipette which is calibrated with mercury

A

Micropipettes

56
Q

No calibration and it For biologic fluid without specific volume

A

Pasteur Pipette

57
Q

Is the most routinely used pipette

A

Automatic Pipette

58
Q

Advantages: automatic pipette

A

–o Time savings
–o Safety
–o Stability
–o Ease of use
–o Increase in precision
–o Lack of required cleaning

59
Q

Tips (contaminated) are often disposabl

A

Automatic pipette

60
Q

Type of automatic pipette uses

A

o Air displacement (no contact with water)
–o Positive Displacement ( work as syringe, tip is not disposable)
–o Displacement Dilutor ( used bacteriology)

61
Q

Should be made of glass that is
resistant to many chemicals used
and resistant to heat

A

Beaker

62
Q

Used for general mixing and
reagent preparation

A

Beaker

63
Q

Wide, straight-sided cylindrical
vessels and are available in many
sizes in several forms

A

Beaker

64
Q

Used to measure volumes of liquids when
high degree of accuracy is not
essential

A

Graduated measuring cylinder

65
Q

pear shaped flasks and Used to contain a specific amount of
volume of liquid

A

Volumetric flasks

66
Q

Precision is up to 1/1000 of a gram

A

Analytical balance

67
Q

Classes of Balance:

A
  1. Number of Pans (Single or Double)
  2. Mechanical or Electronic
  3. Operating Ranges
68
Q

Precision is up to 0.1 gram

A

Rough/platform balance

69
Q

CENTRIFUGE types

A

–Fixed-angle head
–Swinging-bucket type
–Ultracentrifuge
–Tachometer
–Strobe light

70
Q

Is a substance that occurs naturally or is obtained through
a chemical process

A

Chemical

71
Q

Defined as any substances employed to produce a chemical reaction

A

Reagent

72
Q

The following are general description of the various
grades of chemicals available for the clinical
laboratory

A

Grades of chemical

73
Q

These chemicals are of a high degree of purity and
are used often in the preparation of reagents in the
clinical laboratory for many reagent grade or AR
chemicals, and those that meet their standards are
designed by the letter ACS (American Chemical
Society)

A

Reagent Grade / Analytic Reagent (AR) Grade

74
Q

These chemicals are sufficiently pure to be used in many analyses
in the clinical laboratory However, the designation does not reveal
the limits of impurities

A

Chemically Pure (CP) Grade

75
Q

They are generally less pure than CP grade, as the
tolerance is specified such as they are not injurious to
health rather than chemically pure

A

United States Pharmacopia (USP) and National
Formulary (NF) Grade

76
Q

These chemicals are used only for industrial
purposes and are generally not used in the
preparation of reagents for the clinical laboratory

A

Technical / Commercial Grade

77
Q

These agencies or bureaus all supply certified clinical
laboratory standards

A

National Bureau of Standards, College of American
Pathologists and the National Committee for Clinical Laboratory
Standards (NCCLS)

78
Q

Standard: Highly purified chemicals which may be weighed out directly in the
preparation of solutions of selected known concentration

A

Primary standard

79
Q

Standard: A prepared solution whose concentrations is determined by an
analysis of an aliquot amount of the solution using primary
standard and acceptable reference method.

A

Secondary standard

80
Q

It is actually a sample in which the chemical composition and the
physical characteristics stimulate the specimen being analyzed

A

Reference standard/ control material

81
Q

These samples are useful for proficiency testing, inter-
laboratory surveys and in the calibration of reference
materials (commercial) kits and reagent sets

A

Reference standard/ control material

82
Q

Type of water: from distillation process

A

Distilled water

83
Q

Type of water: from ion-exchange process

A

Deionized water

84
Q

Type of water: from ion-exchange process

A

Deionized water

85
Q

Type of water: uses pressure to force water to move
through a semipermeable membrane

A

Reverse osmosis water

86
Q

Type of water: UV light, sterilization, ozone treatment

A

Ultra filtration

87
Q

Type of water: CLSI (Clinical Laboratory Standards
Institute) recommends

A

Reagent grade water

88
Q

Reagent grade water:

A

A. Clinical Laboratory Reagent Water
B. Special R.W.
C. Instrument Feed Water
D. Water supplied by the Method Manufacturer
E. Autoclave and Wash Water
F. Commercially bottled purified water

89
Q

Water Parameters:

A

–Microbiological content
–pH
–Resistivity
–Silicate
–Particulate matter
–Organics

90
Q

Water purity categories: used for the test methods which requires
minimum interferences (e.g. Trace metal, iron and
enzyme analyses)

A

Type 1

91
Q

Water purity categories: acceptable for most analytic requirements
(e.g. Reagent, Q.C, standards preparation)

A

Type 2

92
Q

Water purity categories: autoclave, wash water

A

Type 3

93
Q

Water Filtration:

A

•Glass
• Cotton
• Activate charcoal
• Submicron filters

94
Q

Chemical agents that prevent the coagulation or clotting of blood
are anticoagulants.

A

Anticoagulant

95
Q

Removing the Ca from the blood by precipitation of binding in
unionized form

A

Tri-Sodium Citrate and EDTA

96
Q

Neutralization of thrombin

A

heparin

97
Q

Precipitation of Ca as insoluble salt

A

Oxalate

Cchm Lec (5 decks)

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