Lab One Flashcards
Describe the eyepiece (also known as the ocular).
The lens or lenses on top of the microscope through which the specimen is viewed.
Why do we use oil immersion?
Light travels in a straight line until it meets a new medium; however, different mediums have different speeds with which light travels. When light does not hit at exactly ninety-degrees, it will refract, losing image quality. Oil has a very similar refractive index to glass and thus, reduces refraction.
What kind of epithelium consists of a single layer of flattened cells?
Simple epithelium.
Describe the illuminator.
The illuminator is a low voltage lamp connected to rheostats (varies resistance) to give light of variable intensity.
What kind of tissue forms the framework of the ear?
Elastic cartilage.
What kind of epithelium lines the inside of the urinary bladder?
Transitional epithelium.
What is the main tissue of tendons and ligaments, and forms the protective layer of the eye (sclera)?
Dense connective tissue.
Describe the focusing knobs.
The focusing knobs bring the specimen into focus by moving the specimen closer to or farther from the objectives. There may be separate coarse and fine adjustment knobs on the arm of the microscope.
Describe the sub-stage condenser.
The sub-stage condenser is a glass lens mounted in a metal ring below the stage. It catches diffuse or scattered light rays from the illuminator and focuses them on a specific point.
Describe the stage.
This is the platform with a hole in the middle.
What kind of epithelium has cell nuclei that are located at different levels within cells?
Pseudostratified epithelium.
What kind of epithelium has cells that are longer than they are wide?
Columnar epithelium.
What kind of epithelium consists of many layers of cells?
Stratified epithelium.
What kind of tissue forms the pads of adjacent sections of backbone (vertebrae)?
Fibrocartilage.
Describe the body tube.
Supports the ocular at the top and connects it to the nosepiece and thus, the objectives.
What kind of epithelium has centrally located spherical nuclei?
Cuboidal epithelium.
What kind of cartilage forms the soft part of the nose and has a milk glass appearance?
Hyaline cartilage.
What kind of tissue forms the supporting tissue in the walls of the liver and spleen?
Reticular connective tissue. This kind of tissue is named for the reticular fibres which are the main structural components. This kind of tissue forms the scaffolding for other cells in several organs, including the lymph nodes and bone marrow.
Describe the revolving nosepiece.
It is a circular attachment to the body tube to which different objective lenses are attached.
What kind of tissue is normally arranged around an osteonic canal and has a matrix containing collagen and mineral salts?
Bone tissue.
What kind of tissue transports oxygen and nutrients and fights disease?
Blood.
What kind of tissue functions as a heat insulator, provides stored energy supply, and has its nuclei pushed to the periphery?
Adipose tissue.
What kind of tissue composes some of the body’s largest arteries?
Elastic connective tissue. Elastic connective tissue has limited ground substance and is structured in a flexible but firm matrix.
Describe the objective lens.
These are the lenses which produce the initial real image of the specimen which is further magnified by the ocular lens.
Describe the base.
The base gives mechanical support and a reference point for alignment of optical components.
What kind of tissue binds skin to underlying organs and is also known as the lamina propria?
Loose areolar connective tissue.
Describe the iris diaphragm.
The iris diaphragm opens and closes like an iris to regulate the size of the opening through which light may pass.
Describe the arm.
The arm is the frame of the microscope on which the body tube and revolving nose-piece can be mounted.
