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Hematology 2 > Lab Midterms > Flashcards

Lab Midterms Flashcards

1
Q

Other name for paroxysmal nocturnal hemoglobinuria

A

Marchiafava-Micheli Syndrome

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2
Q

Rare and potentially life threatening disease of the blood characterized by complement induced hemolytic anemia

A

PNH

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3
Q

How is sleeping associated with PNH?

A

Blood pH becomes acidic during sleep which leads to hemolysis of RBC

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4
Q

Screening test in which px RBCs are placed in low-ionic strength solution and observed for hemolysis

A

Sugar or sucrose lysis test

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5
Q

Specific test for PNH

A

Ham’s acid hemolysis

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6
Q

He described the test for PNH in 1937

A

Dr. Thomas Ham

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7
Q

Gold standard, rapid and sensitive test for PNH

A

Flow cytometry

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8
Q

Reagent added in Ham’s acid hemolysis test

A

0.5ml of 0.2N HCl

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9
Q

Another term for sickle cell disease/anemia

A

Drepanocytosis

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10
Q

Genetic blood disorder characterized by abnormal, rigid and sickled RBCs

A

Sickle cell disease/anemia or drepanocytosis

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11
Q

Chemical deoxygenizers

A

2% sodium metabisulfite
2% sodium sodium dithionite
2% ascorbic acid

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12
Q

Best method for sickle cell determination

A

Daland and Da Silva Method

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13
Q

Presence of sickle hemoglobin can be demonstrated with

A

Sickle solubility test

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14
Q

Tests for sickle cell anemia determination

A

Daland and Da Silva

Sherman’s test

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15
Q

Test that follows the law of osmosis, in which there is a transfer of solution from lower to higher concentration

A

EOFT

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16
Q

RBC + hypertonic solution = ?

A

Shrinking/crenating

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17
Q

RBC + hypotonic solution = ?

A

Swelling/bursting

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18
Q

These cells are fragile due to increased osmotic fragility index

A

Spherical cells

19
Q

Resistant cell due to low osmotic fragility index

A

Sickle cell

20
Q

Hemolysis of normal cells begin in ? % salt solution

A

0.39-0.45% salt solution

21
Q

Complete hemolysis of normal cells occur in ? % salt solution

A

0.30-0.33% salt solution

22
Q

This test’s principle is on the stability of red cells under different concentration of hypotonic sodium chloride solutions

A

Sanford method

23
Q

Automated method for EOFT

A

Fragillograph

24
Q

Blood is diluted in hypotonic saline and buffered to pH 7.4 wih phosphate

A

Dacie’s method

25
Q

Conditions that cause increased EOFT

A

Hemolytic anemia

Spherocytosis

26
Q

In the case of spherocytosis, IH occurs at ? and CH at ?

A 
IH = tube 24
CH = tube 20
27
Q

Severe IDA: IH and CH?

A 
IH = tube 19
CH = tube 15
28
Q

Standard factor for concentration of NaCl

A

0.02

29
Q

Protects cell from complement

A

CD59

30
Q

For early complement activation

A

CD55

31
Q

Protein absent in PNH

A

GPI Protein

32
Q

Test for cold hemolysin

A

Donath Landsteiner

33
Q

Cold Ab in Donath Landsteiner is associated with this blood group system

A

P Blood group system

34
Q

What Ig class is a Donath Landsteiner cold hemolysin?

A

IgG

35
Q

Principle of Donath Landsteiner

A

Raynaud’s phenomenon

36
Q

Stain for differentiation of AML from ALL, stains granules black

A

Sudan Black B

37
Q

Helpful in diagnosing erythroleukemia

A

Periodic acid schiff

38
Q

Differentiates myeloblastic and monoblastic leukemias from lymphoblastic leukemias

A

Periodic acid schiff

39
Q

Basic fuchsin dye in PAS

A

Schiff’s reagent

40
Q

Color of PAS stain

A

Magenta to purple

41
Q

Other term for specific esterase

A

Naphthol AS-D Chloroacetate

42
Q

Granules of myeloblasts stain blue-black
Stains neutrophilic granulocytes
Differentiates monoblasts and myeloblasts

A

Specific esterase

43
Q

Other term for nonspecific esterase

A

Alpha-Naphthyl Acetate

44
Q

Added to inhibit activity of nonspecific esterase in monocytes

A

Na fluoride

Hematology 2 (2 decks)

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