Lab Final Flashcards
what chromosome contains the apterous mutation?
chromosome 2
what chromosome is the white eye mutation found on?
chromosome 1st
what is the expected phenotypic ratio of a dihybrid heterozygous crossed with a homozygous recessive?
1:1:1:1
what type of hydroponic system did we use to grow radish plants?
water culture system
what gender are sex-linked disorders more common in?
males
what is a node?
the position where one branch splits off into two
why were the stems cut right below the node?
right below the node is where the most cells are needed to create meristem tissue
what are two advantages hydroponics has over traditional plant growth in soil?
- can be grown year round in greenhouses
2. no pesticides/heribicdes
what is the phenotype of apterous flies?
no wings
What compounds are necessary for a PCR reaction?
Taq polymerase, primers, buffer, dNTPs, MgCl2, H2O,
What primer sets were in the GMO experiment?
Tubulin and 35s
What stage of development must the flies be in order to dissect and obtain a good salivary gland chromosome?
Larvae
Why are drosophila melanogaster a good model organism?
- Short generation time
- Small and easy to keep in laboratory
- Produce good numbers of offspring
- have a chromosome number of 8
- Easily viewable genetic characteristics
Why do ewe target politeness chromosomes?
Because the DNA duplicates but stays attached to each other giving us thousands of double stranded DNA molecules
what primer sets were used in the GMO lab?
tubulin and 35s
What do the dark areas of ploy ten chromosomes represent following staining?
Tightly binded DNA
what is the purpose of cold ethanol when purifying DNA?
to precipitate the DNA
what is the purpose of CaCl in bacterial transformaiton?
it precipitates the DNA
what is the purpose of protease during DNA purification?
to break down the membrane
what is NaOH used for?
to denature the DNA in single strands
how can the GFP gene be used as a marker gene?
it gets inserted into the target bacteria and will cause the it glow green indicating the transformation was successful.
what is the purpose of the heat shock?
it facilitates the entry of DNA into the bacterial cells
what is the purpose of the recovery period following the heat shock in bacterial transformations?
it allows the bacteria to repair their cell walls and express the antibiotic resistance gene
what must the samples be kept on ice prior to inserting them into the PCR machine?
to prevent non-specific binding
why were the plates incubated at 37 degrees Celsius during the bacterial transformation lab?
to allow the bacteria to recover from the heat shock
What is the purpose of tubulin?
It is in all plants so it provides us with a positive control
