Lab Final

Question 0

what chromosome contains the apterous mutation?

Answer 0

chromosome 2

Question 1

what chromosome is the white eye mutation found on?

Answer 1

chromosome 1st

Question 2

what is the expected phenotypic ratio of a dihybrid heterozygous crossed with a homozygous recessive?

Answer 2

1:1:1:1

Question 3

what type of hydroponic system did we use to grow radish plants?

Answer 3

water culture system

Question 4

what gender are sex-linked disorders more common in?

Answer 4

males

Question 5

what is a node?

Answer 5

the position where one branch splits off into two

Question 6

why were the stems cut right below the node?

Answer 6

right below the node is where the most cells are needed to create meristem tissue

Question 7

what are two advantages hydroponics has over traditional plant growth in soil?

Answer 7

1. can be grown year round in greenhouses

2. no pesticides/heribicdes

Question 8

what is the phenotype of apterous flies?

Answer 8

no wings

Question 9

What compounds are necessary for a PCR reaction?

Answer 9

Taq polymerase, primers, buffer, dNTPs, MgCl2, H2O,

Question 10

What primer sets were in the GMO experiment?

Answer 10

Tubulin and 35s

Question 11

What stage of development must the flies be in order to dissect and obtain a good salivary gland chromosome?

Answer 11

Larvae

Question 12

Why are drosophila melanogaster a good model organism?

Answer 12

1. Short generation time
2. Small and easy to keep in laboratory
3. Produce good numbers of offspring
4. have a chromosome number of 8
5. Easily viewable genetic characteristics

Question 13

Why do ewe target politeness chromosomes?

Answer 13

Because the DNA duplicates but stays attached to each other giving us thousands of double stranded DNA molecules

Question 14

what primer sets were used in the GMO lab?

Answer 14

tubulin and 35s

Question 15

What do the dark areas of ploy ten chromosomes represent following staining?

Answer 15

Tightly binded DNA

Question 16

what is the purpose of cold ethanol when purifying DNA?

Answer 16

to precipitate the DNA

Question 17

what is the purpose of CaCl in bacterial transformaiton?

Answer 17

it precipitates the DNA

Question 18

what is the purpose of protease during DNA purification?

Answer 18

to break down the membrane

Question 19

what is NaOH used for?

Answer 19

to denature the DNA in single strands

Question 20

how can the GFP gene be used as a marker gene?

Answer 20

it gets inserted into the target bacteria and will cause the it glow green indicating the transformation was successful.

Question 21

what is the purpose of the heat shock?

Answer 21

it facilitates the entry of DNA into the bacterial cells

Question 22

what is the purpose of the recovery period following the heat shock in bacterial transformations?

Answer 22

it allows the bacteria to repair their cell walls and express the antibiotic resistance gene

Question 23

what must the samples be kept on ice prior to inserting them into the PCR machine?

Answer 23

to prevent non-specific binding

Question 24

why were the plates incubated at 37 degrees Celsius during the bacterial transformation lab?

Answer 24

to allow the bacteria to recover from the heat shock

Question 25

What is the purpose of tubulin?

Answer 25

It is in all plants so it provides us with a positive control