Lab Exam Flashcards
Primates
Entamoeba histolytica
Ameoba pathogenic to primates
Infection in liver and brain can be fatal
Four (4) nuclei is diagnostic
Human
Entamoeba coli
Eight nuclei
Larger size than E. histolytica
NON-pathogenic
All animals + humans
Naegleria fowleri
Large dark nuclear endosome is diagnostic
Brain infection from swimming in hot water
Infects all animals + humans
Animals + humans
Giardia sp.
Trophozoite stage (rare in feces)
Tear drop shap and two nuclei
Remember zinc sulfate soolution
Animals + humans
Giardia sp.
Cyst stage
Four nuclei (only 3 may be visible)
More common in feces
Cattle
Trichomonas foetus
Undulating membrane is diagnostic
Direct smear of preputial wash from bull
Causes abortions in cattle
Chickens & turkeys
Histomonas meleagridis
Round parasite with central nuclei
Large space between parasite and host = diagnostic
Infects turkeys
Heterakis is a transport host (nematode)
Swine and humans
Balantidium coli
Trophozoite stage
Harmelss commensal of swine intestine
Pathogenic in humans (primates)
Pear shaped
Macro and micro nulcei plus cilia
Swine and humans
Balantidium coli
Cyst stage
Fish
Ichthyophthirius multifiliis
Trophozoite stage
Horse-shoe macromucleus
“White spot” in fish
T. stage lives in galleries in the epithelium
Fish
Ichthyophthirius multifiliis
Infective stage = tomite or swarmer
Red stained both macro and micro nucleui
Rabbits
Nosema cuniculi
Dark stainging spores!
Gram positive staining
Infects rabbits
Chickens
Eimeria tenella
Major coccida of chickens
Infection of cecum
Note: Schizonts containing merozoites (bananas)
Chickens
Eimeria tenella
Chicken cecum
Note: bloody casts
Cattle
Eimeria bovis
Left: unsporulated oocyst
Right: sporulated oocyst
4 sporocysts with 2 sporozoites each
Highly pathogenic bovine coccidia
Goose kidney
Coccida
Eimeria truncata
Infects goose kidney
Ocysts in urine!
Rabbits
Eimeria stiedai
Infects rabbits
Swine
Coccidia
Isospora suis
Most pathogenic coccida of swine
2 sporocysts with 4 sporozoites each!
Eimeria versus Isospora
Eimeria: cattle and poultry
4/2
4 sporocysts with 2 sporozoites each
Isospora: dogs and cats
2 sporocysts with 4 sporozoites each
Swine and humans = both are infective
Mouse abdominal fluid
Toxoplasma gondii
Motile tachyzoites
DH: cat
Mouse brain
Toxoplasma gondii
Cyst with bradyzoites
Duck muscle
Sarcocystis sp.
Rice grains in muscle = sarcocysts
Duck is IH
DH are carnivores
Thin walled sporulated sporocyst in fecal float
All mammals
DMSO carbol fuschin stain
Cryptosporidium parvum
Pink stained oocysts
Small oocysts
4-8 naked sporozoites in fresh feces = NO sporocyst
Does not need to sporulated to be infective
All mammals
Cryptosporidium parvum
Oocysts on villi of small intestine
Humans
Plasmodium vivax
Human malaria
Ring stage in RBC with jewel and ring
Birds
Haemoproteus columbae
Pigeon
Blue staining on one side of RBC
Turkey
Leucocytozoon smithi
Parastized WBCs
Transmitted by black flies (fast water)
Turkey
Leucocytozoon sp.
Cracked appearance of cytomeres in the schizont
= Megaloschizonts
Black fly (Simulium sp.) in the vector
Dogs
Babesia canis
Tear-drop shape in RBCs
Vector: brown dog tick
Bovids
Theileria parva
Pleomorphic: varies in shape
Infects cattle (Africa)
DH: Ixodid (hard) ticks
Bovids
Theileria parva
Koch’s blue bodies = schizonts of Theileria
Inside macrophages
What characterizes flukes?
- Flat
- Suckers (acetabula)
- Blind gut = no anus
- Tegumant
- Hermaphroditic = monoecious
- No body cavity
Cattle, sheep & humans
Fasciola hepatica
Common liver fluke of sheep adn cattle
Cepalic cone = diagnostic
Cattle, sheep & humans
Fasciola hepatica
Large, amber colored eggs
Need fluke finder!
Cattle, sheep & humans
Fasciola hepatica
Miracidia
Penetrates snail (IH)
Cattle, sheep & humans
Fasciola hepatica
Lymnid snails
Intermediate hosts
Drainage ditches
Cattle, sheep & humans
Fasciola hepatica
Cercaria
Emerges from the snail
Encysts as metacercaria on vegetation
White tailed deer
Fasciola magna
NO cephalic cone!
Branched organs
DH: white tailed deer
Cattle & sheep
Dicrocoelium dendriticum
Lancet fluke
Parasitizes bile ducts esp. sheep
WIll NOT float
Not in MN
Cattle & sheep
Dicrocoelium dendriticum
Egg
Mink, dog, cat + humans
Paragonimus sp.
Lung fluke of mink, dog and cat
Mink, dog, cat + humans
Paragonimus sp.
Egg
Dark amber
Flattened on one side
Septum or feces!
Fish eating carnivores (dog)
Nanophyetus salmincola
Salmon poisoning fluke
Harbors Neorickettsia bacteria
Carnivores (dog)
Alaria sp.
Surface of small intestine
Carnivore eats frog or snake
Most common fluke seen in dog feces in MN!!!
Yellow large eggs
Chickens
Prosthogonimus sp.
Fluke of oviduct of fowl
Can show up in chicken eggs!
Birds
Echinostoma sp.
Flukes of birds and mammals
Spiny collar!!!
Birds and humans
Avian Schistosome
Swimmer itch
Cercaria
Fish
Black spot
Fluke metacercaria
Fish = IH
DH = belted kingfisher
No risk to humans
Insects
Horsehair worm
Freeliving stage of insect parasite
NOT nematodes
Pet may vomit after eating grasshopper
Tapeworm characteristics
- Flat
- Suckers
- Segmented!
- No gut
- Tegument
- Hermaphroditic
- No body cavity
Dog and cat
Tapeworms
Cyclophyllidean eggs
Hexicanth larvae are present in egg immediatly
Cats and dogs
Pseudophyllidean eggs
Tapeworms
Look just like fluke eggs when laid
Need time to develop hexacanth larvae
Which cestode life stage is most patogenic?
Larval stage
Humans, bears and dogs
Diphyllobothrium latum
Pseudophyllideans (incubation)
LACKS suckers!
Infects humans, bears and dogs
Risk when eating raw fish
Predator-prey
Taenia sp.
Cyclophyllideans (larvated eggs)
Radially striated shell
Eggs in segments difficult to see on a fecal
Motile rice grains near anus!!!
Predator eats prey with muscle cysts
Predator-prey
Taenia sp. scolex
4 sucker and 2 circles of hooklets = scolex