Lab class one Flashcards
1
Q
fluorescence assay:d escribe chemical used
A
hoescht dye: binds to minor groove, when lit with UV light, shines blue
2
Q
describe how the fluorescence assay was made
A
differing proportions of DNA buffer and DNA were added
the same amount of hoesecht dye was added
standard curve was made of absorbance vs concentration
absorbance of unknown compound was used to determine concentration.
3
Q
what are the 2 types of uncut plasmid DNA
what are the 2 other relevant types of DNA
A
ncked and supercoiled
linear - cut plasmid
single stranded
4
Q
describe where each will show up on electrophoresis
A
TOP: nicked
linear
supercoiled
BOTTOM: single stranded
5
Q
describe relationship between base pairs and motility
A
y axis: bp’s
x axis: mobility
first: linear: 45* down
then, logarithmic: curves down
