Cancer

Question 1

what 3 changes occur in tumourgenesis

Answer 1

immortalisation: cells can divide indefinitely but obey contact inhibition
transformation: cells can divide indefinity but dont obey contact inhibition
metastasis

Question 2

describe c-myc oncogene function

Answer 2

produces a transcription factor which activates genes associated with cell division

Question 3

why must c-myc be carefully regulated

Answer 3

because if uncontrolled, then there’ll be unregulated activation of cell division genes leading to uncontrolled cell div, potentially cancer

Question 4

describe burkitt’s lymphoma

Answer 4

chromosome 8 has C-myc
chromosome 14 has enhancer for heavy chain of antibody
translocation occurs, so c-myc goes under enhancer on chromosome 14

ehancer causes constant expression of C myc, hence constant/uncontrolled division of B cell, hence B cell lymphoma

Question 5

how can burkitt’s lymphoma be detected

Answer 5

PCR:
J-trans-coding
put primer on D and coding
then separate by electrophoresis