LAB BLOOD WORK

Question 1

PCV

Answer 1

% of whole blood composed of RBC

• EDTA or HEPARIN tube (anti coagulant)
• 3/4 full
• more accurate bc centrifuged

Question 2

RBC

Answer 2

have the highest specific gravity, and gravitate bottom tube

Question 3

plasma protein

Answer 3

same things needed as PCV

• use refractometer
• break tube just ABOVE buffy coat
• g/dl

Question 4

Erythrocyte indices

Answer 4

classification of certain anemia

• MCV
• MCH
• MCHC

Question 5

MCV

Answer 5

average size of erythrocyte

Question 6

MCH

Answer 6

average weight of hemoglobin contained in average RBC

Question 7

MCHC

Answer 7

concentration of hemoglobin in average erythrocyte

g/dl

Question 8

serum

Answer 8

plasma from fibrin,plasma protein, has been removed

Question 9

hemolysis

Answer 9

ruptured cel

Question 10

PLASMA PROTEIN

Answer 10

produced primarily by liver and immune system

-liver, and kidney disease (most common)

Question 11

total protein

refractometer

Answer 11

-very helpful hydration status
dehydrated=elevated protein
overhydrated=decreased protein

Question 12

albumin

Answer 12

most important protein
35% - 50% of total plasma protein in most
LIVER, RENAL DISEASE

Question 13

ALBUMIN

Answer 13

major building and tranport protein in blood

-maintain osmotic pressure in plasma

Question 14

FIBRINOGEN

Answer 14

-synthesized 4 hepatocytes
-forms matrix of blood clot
-necessary for blood clots
inflamation, tissue damage elevate

Question 15

fibrinogen

Answer 15

plasma only sample used
serum does not contain fibrinogen
EDTA

Question 16

composition of blood

Answer 16

!. erythrocyte (RBC) bone marrow, HGB
carry O2 and waste from cells/tissue
2. leukocyte (WBC)
Bone marrow(stem cells) circulate blood/lymph 5 types MEN LB body army
3. thrombocyte (plates)
made by megarocyte,jagged edge clot blood
4. plasma
straw color, electrolyte, hormones
maintain PH/temp.
5. serum
plasma w/out fibrinogen NO anticoagulant,fluid on top is serum

Question 17

complete blood count

CBC

Answer 17

doesnt measure specific organ function!
measure over all health!
degree anemia

Question 18

PCV is also known as?

Answer 18

microhematic or hematocrit

Question 19

HCT is?

also known as PCV

Answer 19

circulation by an automated instrument

-still get %

Question 20

total protein

LIVER, break down protein

Answer 20

measures protein concentration in the blood
-hydration status
-ESTIMATE
-EDTA!!
PLASMA must be CLEAR!!!
HIGH results=LIPEMIC

Question 21

INCREASE/DECREASE in TP means?

Answer 21

elevated TP=dehydration

decrease TP= OVER hydration

Question 22

common proteins

Answer 22

albumin
globuliin
fibrinogen

Question 23

albumin

Answer 23

most important
35-50% TP in most animals
osmotic pressure

Question 24

globulin

Answer 24

antibody production

binding molecule

Question 25

Fibrinogen

Answer 25

synthesized in the liver precursor 4 fibrin to aid in clotting 3-6% TP content NOT in SERUM

Question 26

what do you use to test TP

Answer 26

``` hematocrit tube you used for PCV -break tube in half between buffy coat and plasma -discard clay end -refractometer G/dl ```

Question 27

HGB | hemoglobin concentration

Answer 27

main component RBC | outside lab most accurate

Question 28

RBC | red blood cell count

Answer 28

``` 3 methods 1. automated (most accurate) 2. manual unopet or hemacytometer rare to do cant get solution 3. estimation mathmateical ```

Question 29

RBC normals

Answer 29

``` canine = 5.8 - 8.5 feline = 5.0 - 10.0 ```