Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology Lab > Lab Bacterial culture media > Flashcards

Lab Bacterial culture media Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

What is the standard for autoclave conditions?

A

121C for 15 minutes at 15 psi.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How do you know if the medium was contaminated before inoculating it with your cultures?

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the role of agar?

A

Agar acts as a solidifying agent (melts at 100c and solidifies at 44C)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Slective

A

permits growth of desired organisms and selects against undesired organisms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Differential

A

permits growth of many different types of organisms but allows you to differentiate them based on their appearance.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Enrichment

A

contains nutrients that enhance the growth of a desired organism.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

General media

A

supports the growth of most bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

MacConkey’s Agar (MAC)

A

Selective- inhibit growth of Gram-positive bacteria (selects gram-negative)
Differentiates- between lactose fermenters (pink colonies) and non-fermenters (no color change)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Mannitol Salt agar (MSA)

A

Selective- selects for staphylococcus species (high salt concentration)
Differential (mannitol)- differentiates between mannitol fermenters (yellow colonies) and non-fermenters (no color change)
Phenol red (pink-basic/yellow-acidic)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Phenylethyl alcohol agar (PEA)

A

Selective- selects for gram-positive (phenylethyl alcohol)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Eosin methylene blue agar (EMB)

A

Selective-selects for gram-negative.
Differential- differentiates between strong lactose fermenters (green metallic colonies), weak lactose fermenters (pink colonies) and non-fermenters (no color change)
Enrichment (lactose/sucrose)-enhances growth of fecal coliforms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Blood agar

A

Enrichment (Blood)- enhances growth of fastidious bacteria (pathogens).
Differentiates- differentiates based on type of hemolysis. Alpha-hemolysis (partial destruction of RBCs, greenish color may develop around colonies), Beta-hemolysis (complete destruction of RBCs, zone of clearance surrounds colony) and y-hemolysis (non-hemolytic/no change)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

List essential macronutrients.

A

C, N, O, H, P, and S.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

List essential micronutrients.

A

Mg, Fe, K, Ca, and Zn.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

How much dry media powder will be used to prepare 250ml of TSA?

A

Given 250ml and 40g
250/2= 0.25L
40g/2=10g

How well did you know this?
1
Not at all
2
3
4
5
Perfectly

Microbiology Lab (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions