Lab 1 Flashcards
What type of media would you use to allow growth of many bacterial species from the sampled environment?
Tryptic Soy Agar (TSA)
Where would you incubate a soil sample?
You would incubate a soil sample at 25C.
Where would you incubate a Mouth/touch plate? Sample from frozen tundra? Why
You would incubate mouth and touch plate at 37C.
Proper sterilization of the inoculating loop before and after use
The inoculating loop is sterilized before and after use to prevent contamination of unwanted organisms.
Transferring cultures from liquid-to-plate or liquid-to-liquid.
Transferring cultures from liquid to plate.
Define resolution
The ability to distinguish two small objects apart from each other.
Define magnification
The ability to make small objects appear larger.
Define parfocal
The focal points of each lens are the same approximate point.
List the objective lenses:
Scanning 4X
Low power 10X
High power 40X
Oil immersion 100X
Eyepiece of a brightfield microscope?
10X
What is the total magnification?
The objective lens and the ocular lens (ex: 4 X 10= 40x)
What is the proper way of handling a microscope?
What is simple stain?
What is the most common stain?
What are the basic stains used?
Crystal violet, safranin, and methylene blue.
Basic stains have a positive charge, True or false?
True basic stains have a positive charge that binds to the cell surface and stain the bacteria.
What are acidic stains used?
Nigrosin
Acidic stains have a negative charge, True or False?
True and stain the background of the cell.
What is differential stain?
Purpose of heat-fixing after the smear has air-dried.
Heat fixing helps to make sure the cells sticks to the slide.
Who discovered Gram stain?
Hans Christian Gram
How are bacteria differentiated? What morphological feature?
What are the reagents used for the gram stain?
