Lab Act 2 Microscopy Reading Flashcards
-It is a precise and delicate instrument consisting mainly of a system of lenses, assembled to give a sharp, distinct and magnified image of the microscopic specimens.
- important tool in the study of Biology
Microscope
What are the parts of the compound microscope
-Magnifying/Optical Parts
-Illuminating Parts
-Mechanical Parts
-Focusing Mechanism
are composed of ground and polished lenses for the enlargement of the image and objects under study.
Magnifying/Optical Parts
Are found below the stage for light gathering and regulation
Illuminating Part
microscope consist of precise parts of metals to support and adjust the optical parts.
Mechanical Parts
used to raise or lower the stage to get the right image of the specimen.
Focusing Mechanism
which are attached to the revolving/rotating nosepiece that may be of four kinds. It gives the initial magnification.
Objectives
is found on top of the microscope which gives the final magnification of the specimen with a magnifying power of 10x.
Ocular/Eyepiece
is the shortest objective usually marked 4x. It provides general view of the specimen.
Scanner
a shorter objective usually with a magnifying power of 4x to 10x with a working distance of 16mm. It also provides the general view of the specimen.
Low Power Objective (LPO)
is usually marked 40x to 60x with a working distance of 4mm. It provides a more detailed view than the LPO.
High Power Objective (HPO)
gives the highest magnification with a magnifying of 97x to 100x with a working distance of 1.8mm
Oil Immersion Objective
illuminates the object to be seen. The source can either be a mirror or the instrument is self-illuminating. Mirror has two reflecting surfaces; plane and concave. Plane surface of the mirror is used when the source of light is artificial but when natural, the concave surface of the mirror is used.
Light source
found below the stage connected to the condenser is used to regulate the amount of light by means of a lever.
Iris Diaphragm
found below the stage is used to concentrate light to the specimen under study.
Condenser