Lab 8 Quiz (for 10/30)

Question 1

What is the hills peak name?

In diagram

Answer 1

Activation energy

Question 2

Definition:

Activation Energy

Answer 2

energy required to start the reaction

(thermal energy)

Question 3

Definiton

Catalyst

Answer 3

substance that speeds up a reaction by lowering the activation energy without being altered

Question 4

Definition:

Enzyme

Answer 4

a macromolecule that acts as a catalyst

Question 5

What type of macromolecule is an enzyme?

Answer 5

Protein

Question 6

What do enzymes do?

Answer 6

it just speeds up the rate of the reaction

Question 7

What are the steps of enzymes?

Hint: 5

Answer 7

1. Active site on enzymes

Question 8

Are enzymes proteins?

Answer 8

Yes
Most of them

Question 9

What affects enzymes?

Answer 9

Temperature
pH

Question 10

Enzyme used in lab 8

Answer 10

Amylase

Question 11

Substrate for amylase is
(in lab 8)

Answer 11

starch

Question 12

Indicator for starch?

Hint: IKI

Answer 12

Iodine Potassium

Question 13

In Spectrophotometer

If we have a lot of starch =

Answer 13

HIGH light absorbance
- closer to 1

Enzyme is not doing a good job!

Question 14

In Spectrophotometer

does not have a lot of starch =

Answer 14

LOW light absorbance
- closer to 0

Enzyme is doing it’s job!

Question 15

On lab:

pH and Absorbance

Answer 15

absorbance decreased as pH increased
- likes more basic #’s for best enzyme activity

Question 16

On lab:

Time and Absorbance

Answer 16

Absorbance decreased as time increased
- starch is breaking down more as time increases

Question 17

On lab:

Temperature and Absorbance

Answer 17

• 25 degrees works best to break starch down

Question 18

On lab:

Tube (increasing of enzyme) and Absorbance

Answer 18

As tube (enzyme) increases the absorbance decreases (it breaks it down)

Question 19

On quiz: graphing questions
how enzymes work