Lab 8 Quiz (for 10/30) Flashcards
Membrane Structure and Transport
What is the hills peak name?
In diagram
Activation energy
Definition:
Activation Energy
energy required to start the reaction
(thermal energy)
Definiton
Catalyst
substance that speeds up a reaction by lowering the activation energy without being altered
Definition:
Enzyme
a macromolecule that acts as a catalyst
What type of macromolecule is an enzyme?
Protein
What do enzymes do?
it just speeds up the rate of the reaction
What are the steps of enzymes?
Hint: 5
- Active site on enzymes
Are enzymes proteins?
Yes
Most of them
What affects enzymes?
Temperature
pH
Enzyme used in lab 8
Amylase
Substrate for amylase is
(in lab 8)
starch
Indicator for starch?
Hint: IKI
Iodine Potassium
In Spectrophotometer
If we have a lot of starch =
HIGH light absorbance
- closer to 1
Enzyme is not doing a good job!
In Spectrophotometer
does not have a lot of starch =
LOW light absorbance
- closer to 0
Enzyme is doing it’s job!
On lab:
pH and Absorbance
absorbance decreased as pH increased
- likes more basic #’s for best enzyme activity
On lab:
Time and Absorbance
Absorbance decreased as time increased
- starch is breaking down more as time increases
On lab:
Temperature and Absorbance
- 25 degrees works best to break starch down
On lab:
Tube (increasing of enzyme) and Absorbance
As tube (enzyme) increases the absorbance decreases (it breaks it down)
On quiz: graphing questions
how enzymes work
