Lab 8: Brucella, Pseudomonas, Burkholderia, Aeromonas, Francisella, Bordetella, Moraxella. Identification of an unknown bacterium II.

Question 1

the Koster - positive bacteria are red?

Answer 1

True

Question 2

we use 5% suphuric acid for Koster staining?

Answer 2

False (we use 0.5%)

Question 3

Brucella melitensis is an obligate aerobic bacterium?

Answer 3

True

Question 4

cattle can be infected by B. ovis

Answer 4

False (sheep)

Question 5

Brucella abortus is a flagellated bacterium?

Answer 5

False (no flagella)

Question 6

Pseudomonas aeruginosa produces two types of pigments?

Answer 6

True

(piocianin, fluorescein)

Question 7

Burkholderia mallei can infect swine?

Answer 7

False

(Burkholderia pseudomallei)

Question 8

Francisella tularensis can infect humans, causing tularaemia?

Answer 8

True

Question 9

Francisella subspecies can be found in rodents and arthropods?

Answer 9

True

Question 10

Bordetella bronchiseptica can cause pneumona in rabbits?

Answer 10

True

Question 11

Brucella abortus is facultative pathogenic bacterium?

Answer 11

False

(Facultative intracellular bacterium)

Question 12

we use 3% safranin in koster staining?

Answer 12

True

Question 13

as a result of koster staining Brucella abortus is Red?

Answer 13

True

Question 14

Brucella suis is obligate anaerobic bacterium?

Answer 14

False

(Obligate aerobic)

Question 15

Brucella suis biotype 2 can infect european brown hares?

Answer 15

True (and wild boar)

Question 16

Glanders (mallei) is caused by burkholderia pseudomallei?

Answer 16

False

(Burkholderia mallei)

Question 17

Pseudomonas aeruginosa is flagellated bacterium spp.

Answer 17

True

1 polar flagellum

Question 18

Burkholder pseudomallei can infect humans?

Answer 18

True

Question 19

Francisella tularensis need cystine or cystin for culturing?

Answer 19

True

Question 20

Aeromonas Spp. is obligate aerobic bacteria?

Answer 20

False

(aerobic and facultative anaerobic )

Question 21

Brucella spp. can be found in infected animals and their direct environment only?

Answer 21

True

Question 22

Brucella suis has 5 biotypes?

Answer 22

True

Question 23

Brucella abortus is a koster positive species?

Answer 23

True

Question 24

B. melitensis, B. abortus and B. canis have strong relationship in antigenic structure, so they can show cross reactions?

Answer 24

False

(B. melitensis - B. abortus - B. suis, cross reactions)

( B. ovis and B. canis cross reactions)

Question 25

Pseudomonas aeruginosa can cause septicaemia in fur animals

Answer 25

True

Question 26

Pseudomonas species are obligate aerobic bacteria?

Answer 26

True

Question 27

Humans are susceptible to burkholderia mallei?

Answer 27

True

Question 28

Optimal temperature for culture of aeromas hydrophilia is above 28 degrees celsius?

Answer 28

False

(optimal temperature is 28 degrees celsius)

Question 29

Francisella turarenis is the causative agent of tularaemia?

Answer 29

True

Question 30

Moraxella ovis - we can see paired cocci on the smear?

Answer 30

True

Question 31

Brucella species are obligate pathogenic bacteria?

Answer 31

True

Question 32

B. suis is a koster negative species?

Answer 32

False

Question 33

During koster staining 3% safranin is used as counter staining?

Answer 33

True

Question 34

B. ovis is a zoonotic agent?

Answer 34

False

Question 35

B. mallei is a widespread bacterium in our environment?

Answer 35

False

Question 36

B. pseudomallei is a flagellated bacterium?

Answer 36

True

Question 37

A. salmonicida can cause furunculosis in fish?

Answer 37

True

Question 38

Cystine or cysteine is used as an additive for culturing of F. tularensis?

Answer 38

True

Question 39

Moraxella ovis can cause pneumonia in calves and sheep?

Answer 39

True

Question 40

Brucella species is able to multiply in the environment?

Answer 40

False

Question 41

Brucella species is obligate anaerobic bacteria?

Answer 41

False

(obligate aerobic)

Question 42

Pseudomonas causes otitis externa in rabbits?

Answer 42

False

(in dogs, and sometimes cats)

Question 43

B. pseudomallei is not a flagellate bacterium?

Answer 43

False

(have flagella)