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Bacteriology > Lab 7: Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella > Flashcards

Lab 7: Pasteurella, Mannheimia, Bibersteinia, Ornithobacterium, Riemerella, Actinobacillus, Haemophilus, Histophilus, Avibacterium, Taylorella Flashcards

1
Q

Genera within Pasteurellaceae family?

A
  1. Pasturella
  2. Mannheimia
  3. Bibersteinia
  4. Actinobacillus
  5. Haemophilus
  6. Avibacterium
  7. Histophilus
  8. Gallibacterium
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2
Q

other genera resembling pasteurellaceae?

A
  1. Ornithobacterium
  2. Reimerella
  3. Taylorella
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3
Q

general habitat of pasteurellaceae?

A

Mucous membranes

(respiratory tract, genitals, alimentary tract)

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4
Q

general morphology of pasteurellaceae?

A

0.5-2μm coccoid rods

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5
Q

general staining of pasteurellaceae?

A
  • Gram - negative
  • Bipolar staining
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6
Q

what is bipolar staining?

A

more intense staining in the middle of the bacteria

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7
Q

what is the general culture of pasteurellaceae?

A
  • Fastidious (Yeast extract, blood, PH-sensitivity)
  • aerobic - facultative anaerobic
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8
Q

general biochemistry for pasteurellaceae?

A
  • oxidase +
  • catalase +
  • Fermentitative
  • toxin production by some species
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9
Q

Pasteurellaceae antigens?

A

cell wall and capsule antigens

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10
Q

pasteurellaceae resistance?

A

Generally weak resistance

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11
Q

Pathogenicity of pasteurellace genera?

A

Facultative pathogens

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12
Q

habitat of pasteurella genera?

A

mucous membranes of the respitatory tract

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13
Q

pasteurella genus produces a capsule?

A

True

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14
Q

colony types of pasteurella?

A

M-S-R colonies

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15
Q

types of cyclotoxins produced by pasteurella?

A

P. multocida D - dermonecrotoxin

  • causing damage to osteoblasts
  • biochemical differenced between P. multocida spp.
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16
Q

P. multocida antigens?

A
  • cell wall antigens
    • 16 Heddleston system
    • (11: namioka system)
  • capsule antigens
    • A,B,E,D,F
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17
Q

species of Pasteurella?

A
  1. P. Multocida A,D
  2. P. multocida D
  3. P. multocida B,E
  4. P. pneumotropica
  5. P. aerogenes
  6. P. canis, P. dagmatis, P. stomatis
  7. P. Caballi
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18
Q

Effects of P. multocida A,D

A
  • Ruminant and swine pneumonia
  • Rabbit septicaemia and pneumonia
  • Fowl cholera
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19
Q

what are the effects of P. multocida D?

A

Atropic rhinitis of swine

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20
Q

what are the effects of P. multocida B,E?

A

Haemorrhagic septicaemia

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21
Q

Habitat of Mannheimia, Biebersteinia?

A

Mucous membranes (respiratory tract)

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22
Q

Mannheimia and Biber steinia cause ß haemolysis?

A

True

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23
Q

toxins produced by mannheimia and biersteinia?

A

leukotoxin production

  • damage of macrophage and endothelial cells
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24
Q

antigens of mannheimia and biersteinia?

A
  • call wall antigens cannot be used for diagnostics
  • capsule
    • M. haemolytica 12 types
    • B. trehalosi 4 types
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25
Q

pathogenicity of mannheimia and Beibersteinia?

A
  1. M. Haemolytica
    • Pasteurellosis
      • ruminant pneumonia
      • Lamb septicaemia
      • mastitis
      • arthritis
  2. B. Trehalosi
    • acute systemic pasteurellosis
      • feed-lot lambs
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26
Q

Actinobacilllus habitat?

A

Alimentary tract, respiratory and genital tract, mucous membranes

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27
Q

Species of Actinobacillus?

A
  1. A. lignieresii
  2. A. equuli
  3. A. suis
  4. A. seminis
  5. A. ureae
  6. A. Pleuropneumoniae
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28
Q

habitat of A. lignieresii?

A

cattle and sheep oral cavity, rumen and gut

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29
Q

morphology of A. lignieresii?

A
  • 1 - 1.2μm rods
  • filaments
  • sulphur granules in lesions (like actinomycosis)
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30
Q

culture of A. lignieresii?

A
  • aerobic, facultative anaerobic
  • poor growth on nutrient agar
  • sticks to the agar
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31
Q

Antigens of A. lignieressi?

A

Cell wall antigens

  • 6 serogroups
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32
Q

pathogenicity of A. lignieressi?

A

cattle, sheep and swine actinobacillosis

  • Wooden tongue
  • granulocytomas
  • pus containing small, hard yellow to white granules
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33
Q

A. equuli habitat?

A

horse and swine alimentary tract, tonsils, respiratory tract

34
Q

A. equuli has filaments?

A

true

35
Q

A. equuli cultures?

A
  • large viscous colonies
    • some strains can haemolyse
36
Q

subspecies of A. equuli?

A
  1. A. equuli subsp. equuli
    1. A. equuli subsp. haemolyticus
37
Q

both A. equuli subspecies cause which disease?

A

actinobacillosis

38
Q

symptoms of actinobacillosis in the foal?

A

septicaemia, nephritis, pneumonia, arthritis

39
Q

habitat of A. suis?

A

Swine mucous membranes

respiratory and genital tract

40
Q

culture of A. suis?

A
  • Blood agar
  • ß hemolysis
41
Q

pathogenicity of A. suis?

A
  • Swine (grower/adult)
    • pneumonia, septicaemia, metritis, abortion, arthritis
  • Suckling piglet
    • septicaemia
42
Q

habitat of A. seminis?

A

mucous membrane of genital tract of rams

43
Q

A. seminis culture?

A

10% CO2 blood agar

44
Q

A. seminis pathogenicity?

A

facultative pathogenic

inflammation of the accessory glands

epidiymitis

abortion

45
Q

habitat of A. ureae?

A

Human respiratory tract

46
Q

habitat of A. pleuropneumoniae?

A

mucous membranes of the respiratory tract of swine

47
Q

some strains of A. Pleuropneumoniae have fimbria?

A

true

48
Q

culture for A. pleuropneumoniae?

A
  • blood agar, chocolate agar with yeast extract
    • ß hemolysis
49
Q

biotypes of A. pleuropneumoniae?

A
  • Byotype 1: V - factor demand
    • satellitism, NAD, fresh yeast extract
  • Byotype 2: V factor is not needed
50
Q

what is factor V?

A

NAD

51
Q

cytotoxins produced by A. pleuropneumoniae?

A

I - II - III - IV cytotoxins produced

  • damage of macrophages and endothelial cells
52
Q

how many serogroups of antigens does A. pleuropneumoniae have?

A

18 serotypes

53
Q

what is the pathogenicity of A. pleuropneumoniae?

A

swine hemorrhagic - necrotic pleuropneumonia

54
Q

habitat of Haemophilius

A

mucous membranes of human and animals

(respiratory and genital)

55
Q

Haemophilus genus have filaments and sometimes fimbria?

A

true

56
Q

Additives to the culture for Heamophilus?

A
  • Haem (X - factor)
  • NAD (V - factor)
  • some species need 10-15% CO2
57
Q

species of haemophilus?

A

H. influenzae

H. Parasuis

58
Q

features of H. parasuis?

A
  • V factor demand
  • 15 serotypes
  • causes Porcine polyserositis (glasser’s disease)
    • causes severe systemic disease characterised by:
      • Arthritis
      • Meningitis
      • Fibrious polyserositis
59
Q

Habitat of Histophilus somni?

A

Urogenital and respiratory mucous membranes

60
Q

culture requirements for H. somni?

A
  • X, V factor not needed
  • CO2​ demand
61
Q

pathogenicity of H. somini?

A
  • cattle
    • calf pneumonia
    • septicaemia
    • thromboembolic meningoencephalitis (TEME)
    • Abortion
    • Mastitis
  • Sheep
    • epididymitis - orchitis
    • abortion
    • mastitis
    • lamb septicaemia
62
Q

Habitat of Avibacterium?

A

respiratory mucosa

63
Q

Avibacterium have filaments?

A

true

64
Q

culture requirements of Avibacterium?

A
  • some species need CO2
  • V factor demand is variable
65
Q

species of Avibacterium?

A
  1. A. paragallinarum
  2. A. gallinarium
  3. A. Avium
66
Q

features of A. paragallinarum?

A
  • CO2 and factor V are needed
  • 3 serogroups, 9 subgroups
  • Infectious coryzoa of hens
    • ​inflammation of sinuses, nasal discharge and sneezing
    • chicken cold - v. infectious
67
Q

Features of A. gallinarum?

A
  • factor V is not needed
  • mild respiratory disease of hens
68
Q

features of A. Avium?

A
  • Factor V is needed
  • saprophyte, sometimes causes mild respiratory disease in hens
69
Q

habitat of orinithobacterium?

A

upper respitatory tract of some bird species

(chicken and turkey)

70
Q

culture needed for Orinthobacterium?

A
  • blood agar
  • 10% CO2
71
Q

biochemistry of orinithobacterium?

A
  • Catalase -
  • oxidase +
72
Q

pathogenicity of orinithobacterium?

A

turkey, chicken, upper respiratory infection

73
Q

habitat of Riemerella anatipestifer?

A

water fowl upper respiratory ways

74
Q

culture for Riemerella anatipestifer?

A
  • blood agar
  • chocolate agar
  • 10% CO2
75
Q

biochemistry of Rimierella anatipestifer?

A
  • extracellular enzymes
  • proteolytic
76
Q

pathogenicity of Riemerella anatipestifer?

A

anatipestifer disease in waterfowl

77
Q

Habitat of Taylorella equigenitalis?

A
  • genital mucous membranes of the stallion
  • clitoris of the mare
78
Q

Taylorella equigenitalis has filaments?

A

true

79
Q

culture needed for taylorella equigenitalis?

A
  • X and V factors are not needed
  • CO2 demand
80
Q

disease caused by taylorella equigenitalis?

A

CEM: contagious equine metritis

Bacteriology (2 decks)

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