Lab 8 Flashcards
1
Q
What are nucleotides?
A
building blocks of nucleic acids; made up of 3 subunit molecules
2
Q
List the 3 subunit molecules nucleotides are made of
A
Phosphate + Sugar + Base
3
Q
Which is more stable DNA or RNA? why?
A
DNA
OH groups are often the target of chemical attack, this makes DNA much more stable than RNA.
4
Q
What is Transformation?
A
The uptake and expression of DNA by a living cell
5
Q
What is Plasmids?
A
Plasmids are small circular dsDNA molecules that exist apart from chromosomes in host bacterial species
6
Q
DNA Spooling Experiment
A
-
DNA is polar due to its highly charged phosphate backbone.** Its
polarity makes it water-soluble** (water is polar) according to the principle “like dissolves like”. - Ethanol is much less polar than water. Adding ethanol to solution disrupts the screening of charges by water. If enough ethanol is added, the electrical attraction between phosphate groups and any positive ions present in solution becomes strong enough to form stable ionic bonds and DNA precipitation
7
Q
What is Deoxyribonuclease I (DNAse I) ?
A
**Deoxyribonuclease I (DNAse I) is an enzyme isolated from the mammalian pancreas can **breaks the phosphodiester bonds **that connect the nucleotide units in DNA.
