Lab 6 - Light Microscopy

Question 1

Chlamydomonas

Answer 1

single-celled green algae used to study flagellar movement

Question 2

Euglena

Answer 2

found in freshwater ponds; most are photosynthetic

Question 3

Protoslo

Answer 3

medium that slows movement of small organisms

Question 4

how would you describe the background in phase contrast 100x? what color is the background?

Answer 4

metallic; light grey

Question 5

In phase contrast, does having the condensor out of focus affect the uniformity of illumination?

Answer 5

yes, shifting the condensor too far from the stage causes a dark area in the center of the field and a red border appeared around the dark area that appeared.

Question 6

In phase contrast, f the objective and substage are mismatched, what color is the background? 10x objective, substage turret at 40? 40x objective, substage turret at 10 or 100?

Answer 6

dark gray; gray

Question 7

In phase contrast, with the 40x objective and the substage turret set to 10 or 100, how much contrast is there within the specimen?

Answer 7

very little contrast between specimen and background