Lab 5 Flashcards
microscopy and the cell
what is the difference between a light compound microscope and a dissecting microscope?
a compound microscope is used to examine types of cells, micro organisms, and tissues where s the dissecting microscope is used to view larger objects and dissecting.
microscope part: the uppermost lens or series of lenses through which a specimen is views
ocular (eyepiece)
microscope part: connects the ocular to the body tube
draw tube
microscope part: holds the nosepiece at one end and includes the draw tube
body
microscope part: serves as a handle
arm
microscope part: revolves and holds the objectives
nosepiece
microscope part: lower lenses attaches to the nosepiece
objectives
microscope part: use for viewing larger specimens or searching for a specimen; magnifies an object 4x
scanning objective
microscope part: used for coarse and preliminary focusing; magnifies an object approximately 10x
low-power objective
microscope part: used for final and fine focusing; magnifies an object approximately 40x
high-power objective
microscope part: uses the optical properties of immersion oil to help magnify a specimen
oil-immersion objective
microscope part: platform on which slides are placed
stage
microscope part: serves as the source of illumination for the microscope
light source (illuminator)
microscope part: regulates light entering the microscope; usually is controlled by a mechanical lever or rotating disk
iris diaphragm
microscope part: a lens system found beneath the state; used to focus the light on the specimen
condensor
microscope part: used to adjust then microscope on scanning and low power only
coarse-adjustment knob
microscope part: used to adjust the specimen into final focus
fine adjustment knob
microscope part: the supportive portion of the microscope, which rests on the laboratory table
base
definition: after the image is focused with one objective, it should focus with others
parfocal
definition: specific distance from the lens where the specimen can be sharply focused
plane of focus
definition: the thickness of the specimen
depth of field
describe the steps in preparing a wet mount slide
- make sure the slide and coverslip are clean.
- place the specimen in the center of the slide
- add a drop of the liquid in the center of the slide on top of the specimen.
- place the coverslip over at a 45 degree angle to avoid air bubbles.