Lab 4: Routes Of Drug Administration Flashcards
What are the 3 Rs?
Replacement
Refinement
Reduction
How should drugs be administered to mice via the oral route?
Grab the skin over the shoulders firmly with the thumb and middle finger;
stretch the head and neck with the index finger to make the oesophagus straight.
Using a ball-tip needle, feed the needle along the roof of the mouth and toward the right side of the back of the pharynx, and then gently pass down into the oesophagus.
Advance slowly into the stomach. No resistance should be felt
What is replacement?
Replacement: the use of non-animal methods such as cell cultures, human volunteers and computer modelling instead of animals to achieve a scientific aim.
What is refinement?
Refinement: the use of methods that alleviate or minimise potential pain, suffering or distress, and that enhance animal welfare for those animals that cannot be replaced.
What is reduction?
Reduction: the use of methods that enable researchers to obtain comparable amounts of information from fewer animals, or more information from the same number of animals.
How is an drug administered via the IV route for mice?
The principal function of these veins is for thermoregulation. They will dilate when the mouse’s body temperature rises in order to disseminate heat.
Applying heat to the whole animal or locally to the tail can be used to cause venodilation making vascular access easier.
The mouse should be restrained so that its tail is accessible. A 25 gauge bevelled needle is used. The vein is located, the needle inserted by directing the needle into the vein with its bevel pointing upward at an angle of approximately 20 degrees. The needle is inserted slowly visualizing the needle as it enters the vein
What is the righting reflex?
Rodents will try to right themselves when placed in an abnormal position, this is termed their righting reflex.
When under the effects of anasthesia, they will lose this reflex which can be used as a marker of the onset and duration of the drug.
What is the mechanism of action of pentobarbitone?
Pentobarbital binds at the GABAA receptor, increasing the duration of time for which the Cl- ionopore is open, prolonging the post-synaptic inhibitory effect of GABA in the thalamus.
Pentobarbitone is more lipophilic than phenobarbitone and will cross the blood brain barrier more easily, resulting in a faster onset and shorter duration
What is the mechanism of phenobarbitone?
Phenobarbital acts on GABAA receptors, increasing synaptic inhibition.
This can therefore elevate the seizure threshold, reducing the spread of seizure activity from a seizure focus.
Phenobarbital may also inhibit calcium channels, resulting in a decrease in excitatory transmitter release.
The sedative-hypnotic effects of phenobarbital are likely the result of its effect on the polysynaptic midbrain reticular formation, which controls CNS arousal.
What is the main physicochemical difference between pentobarbitone and phenobarbitone that result in their differing effects?
Pentobarbitone is more lipophilic than phenobarbitone. It will have a faster onset of action.
What causes determines the duration of a drug effect?
Whether or not the journey of the drug encounters first pass metabolism. This is where a fraction of the drug is metabolised in the liver before it reaches the receptor.
If a drug does not undergo FPM such as a drug administered via the SC or IV route, it will have a longer duration of action
Does the oral or intraperitoneal route have the longer duration ?
The intraperitoneal route will have a longer duration as while both routes of administration are subjected to first pass metabolism, some of the drug is also likely to be broken down in the stomach in oral administration, so there is less bioavailability thus exerting a shorter duration of effect
Does the intraperitoneal or oral route have the faster onset of action?
The intraperitoneal route as it is closer to the receptors and does not have go through most of the GIT first
What is the magnitude of the effect if drug in a whole animal a function of?
Its concentration at the receptor site.
As this concentration increases, a threshold concentration will be reached where an effect of the drug is detectable.
Further increasing this concentration at the receptor site leads to increased intensity of huge effect
What are the factors affecting the concentration of the drug at the receptor site?
The pharmacokinetic properties Abosrption Distribution Metabolism Excretion