Lab 4-Fundamentals of Microscopy & Cell Structure

Question 1

Light microscope uses a series of ____________, _____________, _____________ to enlarge the image of a specimen through which a beam of light is being passed.

Answer 1

Lenses, mirrors, filters

Question 2

In the compound light microscope, a viewer sees an image that is magnified by two different lenses:

Answer 2

1. Ocular lens (10x magnification)

2. Objective lens (4x, 10x, 40x, 100x)

Question 3

Total magnification=

Answer 3

Ocular magnification x objective magnification

Question 4

Highest total magnification possible in light microscopy:

Answer 4

1000x

Question 5

The minimum distance separating two close-by objects at which they can still be perceived as being two distinct objects.

Answer 5

resolving power

Question 6

Most ______ & _______________ can be viewed using light microscopy.

Answer 6

Cells, organelles

Question 7

____________ microscope shines electrons instead of light to reveal details of very tiny specimen.

Answer 7

Electron

Question 8

The resolution possible from an electron microscope is much ______________ than that from a light microscope.

Answer 8

Greater

Question 9

Iris

Answer 9

Allows more/less light in

Question 10

Eyepiece

Answer 10

Ocular

Question 11

4x

10x

40x

100x

Answer 11

Red

Yellow

Blue

White (also known as the oil immersion lens)

Question 12

Always begin to examine a specimen on the lowest power objective (____)

Answer 12

4x

Question 13

The study of tissues

Answer 13

Histology

Question 14

In wet-mound slides, the specimen remains:

Answer 14

Alive and is not preserved…temporary and practical

Question 15

Large knob on microscope:

Small know on microscope:

Answer 15

Coarse focus

Fine focus

Question 16

The traditional microscope used by biologists to study living cells is the:

Answer 16

Light microscope