Lab 4 - Blood and Bone Marrow Flashcards
what type of cell will have two large nuclear lobes and specific granules with crystalline inclusions in TEM
eosinophils
what type of cell have central nucleolous, eccentric nucleus, and chunks of heterochromatin in TEM
plasma cell
what type of cell will have a strap like nucleus and many rod like specific granules in TEM
neutrophils
what type of cell will show an indented nucleus and no specific granules in TEM
monocyte
what type of cell will have a bilobed nucleus, specific granules that obscure the cytoplasm in LM, large electron dense specific granules in TEM
basophils
what is present in a TEM of a platelet to indicate its not activated
intact microtubules and numerous granules present
what does the blood “ECM” consist of
ground substane (plasma) with the fibre component as virtual or potential fibers; the soluble protein fibrinogen will form insoluble strands of fibrin when blood clots
what type of staining is used for blood and bone marrow
wrights staining
what steps do blood smears not have compared to tissues
not embedding and sectioning, so instead the cells are flattened onto the glass slide and are otherwise whole
what can you use to determine the intensity of staining to ensure you are identifying cells properly
look at the intensity and shade of the RBCs before looking for things like eosinophils to orient yourself with the specific visualization of that specific smear
what are the precursors for all blood cells and immune cells and where is it found
stem cells in the bone marrow
where do you find myeloid (blood cell forming tissue)
it fills the marrow cavities of long bones in fetuses and young animals
how can bone marrow be evaluated
either using aspirates of bone marrow to make a cytosmear and stained with wrights
or in FFPE sections with H+E
what type of cells are band cells a precursor for and when would you see them increased in blood
neutrophils - would see them circulating more if the animal is mounting an inflammatory response to damage or infection
what is the difference between a reticular cell vs a reticulocyte
reticular cell = fibroblast found in lympoid reticular tissue
reticulocyte = immature erythrocyte
how will you be able to tell the difference between eosinophilic granulocyte series cells and erythrocytic series cells at a low magnification
eosinophilic granulocyte series cells = obvious eosinophilic specific granules and lobed nucleus
erythrocytic series = smaller, have compacted basophilic nuclei and small amounts of cytoplasm
what type of cells are common in the loose CT underlying GIT epithelia
lymphocytes and plasma cells
what cell type will you never find circulating in normal blood
plasma cells (derived from B lymphocytes)
what are mast cells similar to and what is their function
they are CT cells very similar to blood basophils and then have a common stem cell in bone marrow
involved in inflammatory and immune responses especially those related to allerges
what is the most common cause of skin cancers of dogs
mast cells prone to neoplasia
what is metachromasia
colour shift when certain basic dyes like toluidine blue attach to molecules in close ordered arrays
what part of mast cells are metachromatic
specific granules of the mast cells
what can be injected into animal for better identification of macrophages and how does it work
colloidal iron - enters tissue in resonse to irritant
