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BioL 251L Lab > Lab 3 > Flashcards

Lab 3 Flashcards

1
Q

Cell

A

Unit of life that makes up all living organisms

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2
Q

Prokaryotic cell

A

A single cell that doesn’t have a nucleus (usually bacteria)

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3
Q

Eukaryotic cell

A

Usually multicellular and has nucleus (any organism that has clearly defined nucleus)

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4
Q

Microscope

A

Equipment that allows us to see small structures

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5
Q

Compound microscope

A

A microscope that has multiple magnifying lenses (like two sets of ocular lenses)

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6
Q

Why should you never use the coarse focus when it’s not in 4X or 10X objective lens?

A

You could damage the lens or slides.

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7
Q

What objective lens should you be in when using coarse focus?

A

Either 4X or 10X

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8
Q

Which objective lens is the longest?

A

100X is the longest and has the smallest glass

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9
Q

Which lens allows the most light to pass?

A

4X

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10
Q

Which objective lens requires oil immersion?

A

100X requires oil immersion because it increases light

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11
Q

How to calculate total magnification?

A

Multiply the ocular lens by the objective lens

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12
Q

How does the size of field change with different objective lenses?

A

As magnification/objective lens increase, depth of field decreases

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13
Q

Why are EMs used?

A

Electronic microscopes are used to give better resolution power to see smaller things that light microscopes are limited to

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14
Q

What determines the theoretic resolution limit of a microscope?

A

The light source and wavelengths of the light determines the resolution limit of a microscope

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15
Q

Advantages of using a light microsope

A

Affordable, easy to use, see in color, see living cells

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16
Q

Disadvantages of using a light microscope

A

Resolution limit is determined by light

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17
Q

Advantages of using an electronic microscope

A

You can see smaller things and is powerful

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18
Q

Disadvantages of using an electronic microscope

A

Expensive and can’t see living things (it will die)

19
Q

Field of view

A

The area visible of a magnified sample that is visible through the ocular lens and the circle of light you see in the microscope

20
Q

Parfocal

A

Objectives that can be changed with minimal or no refocusing

21
Q

Working distance

A

The distance between the specimen and the objective lens

22
Q

Depth of field

A

The distance between the closest and furthest points that are in focus

23
Q

Brightness of field

A

The light-gathering power of the objective

24
Q

EM

A

Electron microscope

25
Q

TEM

A

Transmission Electron Microscope

26
Q

SEM

A

Scanning Electron Microscope

27
Q

Structures of TEM

A

Light source, Electron source, condenser lens, specimen, objective lens, objective lens aperture, intermediate lens, specimen, projector lens, fluorescent screen

28
Q

Structures of SEM

A

Electron source, anode, condenser lens, objective lens, back-scattered electron detector, sample, motorized stage , connects to amplifier and scan generator to computer

29
Q

Unicellular

A

An organism that is only a single cell

30
Q

Aggregate

A

Collection of units or particles (e.g., cells) forming a body or mass. To form such a body or mass

31
Q

Multicellular

A

An organism or part that consists of multiple cells

32
Q

Protist

A

Simple eukaryotic organisms that are neither plants, cells, or fungi.

33
Q

Cell wall

A

A rigid or flexible structure that surrounds some types of cells, providing support, shape, and protection

34
Q

Cell membrane

A

Protects the interior of the cell from the outside

35
Q

Nucleus

A

Usually spherical and transparent and holds the majority of the cell’s genetic information

36
Q

Contractile vacuole

A

A membrane bound organelle that helps maintain water balance in cells that helps remove excess water

37
Q

ER

A

Endoplasmic Reticulum - a continuous membrane system that forms a series of flattened sacs within the cytoplasm

38
Q

Mitochondria

A

Membrane-bound cell organelles

39
Q

Chloroplast

A

The green (chlorophyll pigment), spherical organelles within the cytoplasm

40
Q

Cytoplasm

A

The jelly like liquid that fills the inside of a cell

41
Q

Central Vacuole

A

Membrane-bound sac within cytoplasm that is filled with water and dissolved substances

42
Q

Condenser lens

A

Used to focus the light through the sample

43
Q

What is an example of a protist?

A

An amoeba

44
Q

What does the mitochondria do?

A

Generates most of the chemical energy needed to power the cell

BioL 251L Lab (5 decks)

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