Lab 2B- Acid Fast Staining Flashcards
Kinyoun Stain & Ziehl- Neelson methods
Most bacteria stain Gram (+ or -) , but some bacteria have a different ____ ____ composition which makes them difficult to stain with the Gram stain.
Cell Wall
Bacteria with a high ____ _____ cell wall content stain much better with acid fast staining technique
mycolic acid
T or F: Bacteria that are high in mycolic acid are very common.
False- they are NOT common
Bacteria with high mycolic acid cell wall content are called ___ ___.
Acid- Fast
Which two species are acid fast?
Mycobacteria and Nocardia Species
Some acid fast bacteria have an impact on human disease, what is the most common?
Mycobacteria tuberculosis
Name different types of Mycobacteria (4) & Nocardia (1) human pathogens.
List their main host.
1) M. tuberculosis- human
2) M. leprae- human
3) M. bovis- cattle
4) M. avium- birds(pigeons)
5) N. asteroids- humans
What are the 2 main methods of acid- fast staining procedures used today?
The Ziehl-Neelson method
The Kinyuon method
Which stain can be used to stain the protozoan parasite Cryptosporidia?
Kinyoun Method
What is the Kinyoun staining method?
- It uses carbol fuchsin as the primary stain
- Followed by decolorization w/ acid-alcohol solution
- Counterstain with methylene blue
- DOES NOT REQUIRE HEAT `
What is the Ziehl- Neelson method for staining?
1) Apply primary stain of carbol fuchsin
2) Heat Fix
3. Decolorize with acid alcohol
4) Apply counterstain- methylene blue
Acid- fast bacteria retain the primary dye, carbol-fuschin. What color do they stain?
Pink
Non-acid fast bacteria take up the methylene blue dye and appear what color?
Blue
What is the main difference between the Ziehl Neelson and the Kinyuon method?
Ziehl Neelson uses heat to drive primary stain (carbol fuchsin) into the cell wall.
Kinyuon uses a higher concentration of carbol fuchsin & phenol (wetting agent) as their primary stain.
What is the main purpose for doing the acid- fast stain?
To differentiate bacteria into acid fast group and non-acid fast groups. Used when microorganisms which are not stained by simple or gram stain methods.
