LAB 25: Antimicrobial Susceptibility Tests (Kirby test)

Question 1

what was the main focus of Lab 25?

Answer 1

Antimicrobial Susceptibility Tests (the Kirby test)

Question 2

give a case study summary:

Answer 2

3 patients (A=staph, B= pseudomonas, x=postsurgical negative gram infection) have been ordered an antibiotic sensitivity test to see what will work best in each situation

Question 3

how do you make a good lawn test?

Answer 3

make sure to cover the entire agar (line every inch of the agar)

Question 4

what is the goal of sensitivity test?

Answer 4

To see if bacteria will be sensitive (killed) or resistant (ineffective) to antibiotic or intermediate (meaning it might work, it might not)

Question 5

what is the Zone of Inhibition?

Answer 5

-Area where the antimicrobial inhibits the growth of the organism
-To measure, do the diameter of circle by millimeters then divide to get radius
-Bacteria with no zone=resistant to antimicrobial

Question 6

what are the organisms we used in this lab?

Answer 6

Staphylococcus and Pseudomonas