Lab 2 - Microscopy Flashcards
Which molecule is this? What does it do?
Acridine Orange
Stains DNA (green) and RNA (red)
What molecule is this? What does it do?
Amido Black
Stains collagen and fibrin proteins
What molecule is this? What does it do?
Nitro blue tetrozolium
Used to stain enzymes (histochemistry)
What are these cells and what are they stained with?
They are CHO cell line.
Nuclear DNA is stained blue with a Hoechst stain while anti-tubulin antibodies areon the tubulin proteins
What cell is this and what is it showing?
Human cheek cell - brightfeld (left), fluorescnece microscopy (right)
Nucleus stained with DNA binding dye DAPI (blue) and the cell membranes are stained with DiOC6 (green)
What type of cell is this? What stains are used?
Rat aortic smooth muscle cell
Nuclear DNA is stained blue (Hoechst stain) and the actin is stained red with rhodamine
What are these cells? What are they stained with?
Mouse pancreatic cells
GFP is bound to proteins and can be visualized under UV light
Where is the GFP protein from?
A jellyfish (Aequorea victoria)
What is a flourochrome? What is fluorescein?
An antibody combined with a fluorescent compound. It absorbs blue light and emits a yellow-green light.
What are the different types of electron microscopy?
Transmission electron microscope and scanning
What is the difference between SEM and TEM?
SEM allows 3D visualization because gold coats the specimen and electrons bounce off
TEM allows inside visualization and shows thin slices
What is scurvy caused by?
Deficiency of vitamin C, which is required for collagen formation
What cells are these? What does the green one do?
Yellow = nerve cells; green = astrocyte
Astrocytes provide physical support to nerve cells and clean up debris
