Lab 2

Question 1

How much of the dry weight is proteins in cells

Answer 1

50 %

Question 2

proteins are what?

Answer 2

movers and shakers

Question 3

proteins are polymers of what

Answer 3

amino acids

Question 4

what are amino acids linked to

Answer 4

peptide bonds

Question 5

electrophoresis

Answer 5

is one of the major ways to separate and identify proteins

Question 6

electrophoretic separation

Answer 6

depends in great part on the net electrical charge of proteins (also often relevant are the size and shape of the protein)

Question 7

if it has a negative charge

Answer 7

it will migrate towards the positive pole

Question 8

if it has a positive charge

Answer 8

it will migrate towards the negative pole

Question 9

each protein has at least one

Answer 9

carboxyl group (at its carboxy terminus

Question 10

five amino acids w ionic r groups

Answer 10

aspartic, glutamic, lysine, arginine, and histidine

Question 11

negatively charged when in a protein of high pH (carboxyl group)

Answer 11

aspartic and glutamic acid

Question 12

positively charged when in a low pH (carboxyl group)

Answer 12

lysine, arginine, and histidine

Question 13

when there is no net charge

Answer 13

isoelectric point

Question 14

independent variable

Answer 14

property that you change or manipulate in a systematic way

Question 15

dependent variable

Answer 15

what we are measuring that has changed

Question 16

at pH 4

Answer 16

serum 3 - electrode
cytochrome 11 -electrode
myoglobin 7 -electrode

Question 17

at pH 6

Answer 17

serum 9 +electrode
cytochrome 4 -electrode
myoglobin 1 +electrode

Question 18

at pH 8

Answer 18

serum 9 +electrode
cytochrome 7 -electrode
myoglobin 2 +electrode

Question 19

aspartic acid enters high pH environment

Answer 19

becomes negative and goes towards positive electrode

Question 20

aspartic acid into low pH

Answer 20

negative charge to neutral

Question 21

lysine enters low pH

Answer 21

neutral to positive (goes to negative electrode)

Question 22

lysine enters high pH

Answer 22

positive to neutral (in center)