Lab 2 Flashcards
How much of the dry weight is proteins in cells
50 %
proteins are what?
movers and shakers
proteins are polymers of what
amino acids
what are amino acids linked to
peptide bonds
electrophoresis
is one of the major ways to separate and identify proteins
electrophoretic separation
depends in great part on the net electrical charge of proteins (also often relevant are the size and shape of the protein)
if it has a negative charge
it will migrate towards the positive pole
if it has a positive charge
it will migrate towards the negative pole
each protein has at least one
carboxyl group (at its carboxy terminus
five amino acids w ionic r groups
aspartic, glutamic, lysine, arginine, and histidine
negatively charged when in a protein of high pH (carboxyl group)
aspartic and glutamic acid
positively charged when in a low pH (carboxyl group)
lysine, arginine, and histidine
when there is no net charge
isoelectric point
independent variable
property that you change or manipulate in a systematic way
dependent variable
what we are measuring that has changed
at pH 4
serum 3 - electrode
cytochrome 11 -electrode
myoglobin 7 -electrode
at pH 6
serum 9 +electrode
cytochrome 4 -electrode
myoglobin 1 +electrode
at pH 8
serum 9 +electrode
cytochrome 7 -electrode
myoglobin 2 +electrode
aspartic acid enters high pH environment
becomes negative and goes towards positive electrode
aspartic acid into low pH
negative charge to neutral
lysine enters low pH
neutral to positive (goes to negative electrode)
lysine enters high pH
positive to neutral (in center)
