lab 5 (photosynthesis 1)

Question 1

ethanol (carotene) absorbance)

Answer 1

starts really high (beyond 1.5) dies off. has small peak at 650 and goes back down

Question 2

methanol (chlorophyll b)

Answer 2

starts lower, dies off and stays close to 0. has little up (not much) then back to 0

Question 3

ether (chlorophyll a)

Answer 3

has big shoot up (1.8) then die off. big jump near 1.8 (1.5ish) and back to 0ish

Question 4

does changing the concentration of the pigment substantially alter the position (wavelength) of the peaks

Answer 4

No, wavelength is the same and the absorption is the same. wavelength was no significant

Question 5

does changing the concentration of the pigment substantially alter the height (Absorbance) of the peaks

Answer 5

yes, it did change the height/absorbance of het peaks since there was less pigment concentration

Question 6

what appears to be the relationship between the concentration of pigment and the height of the peaks?

Answer 6

as the concentration decrease the height decreases. linear relationship.

Question 7

of the two (position or height), which would be more useful in identifying a substance?

Answer 7

position/wavelength would be more useful, as those varied. absorbance we wouldn’t be able to tell or differentiate between without position/wavelength

Question 8

What would happen to photosynthesis if chlorophyll were transparent to light?

Answer 8

it wouldn’t be able to go through photosynthesis. the absorption would be flat, no energy would be captured.

Question 9

How does chlorophyll relate to the absorption spectrum?

Answer 9

green is not absorbed but reflected which is why photosynthesis happens. other colors absorb the red and blue lights, but do not grow as effectively.

Question 10

what property of chlorophyll and carotenoids did we take advantage of to separate them?

Answer 10

solubility was used to separate the different pigments