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MICROBIOLOGY LAB > LAB 2 > Flashcards

LAB 2 Flashcards

1
Q

Used to observe minute organisms

A

MICROSCOPE

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2
Q

Functions of Microscopes in Microbiology

A
  1. Identify the features of the microorganisms
  2. Characterize microorganisms
  3. Determine the Biology of Microorganisms
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3
Q

has 2 lenses and uses visible light

A

COMPOUND MICROSCOPE

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4
Q

Objectives of the compound Microscope

A

SCANNER - 4X
LPO - 10X
HPO - 40X
OIL IMMERSION OBJECTIVE - 100X

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5
Q

Magnification of ocular lenses

A

5x AND 10x

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6
Q

Formula for calculating the total magnification

A

ocular x objectives

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7
Q

STAINED AND UNSTAINED SLIDES

A

COMPOUND AND ELECTRON MICROSCOPE

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8
Q

Illuminates a dark background; has a special type of condenser that scatters light

A

DARK FIELD MICROSCOPE

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9
Q

Uses beam of light/electrons; Provides a high resolution of biological and non-biological samples

A

ELECTRON MICROSCOPE

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10
Q

uses UV light; specific fluorescent dye; stained

A

FLUORESCENCE MICROSCOPE

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11
Q

Observing live organisms in their natural state; unstained and has a special condenser

A

PHASE CONTRAST MICROSCOPE

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12
Q

Illuminating parts

A

Iris Diaphragm
Condenser
Illuminator

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13
Q

Uses coarse adjustment (maximized movement)

A

LPO and scanning

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14
Q

Uses Fine adjustment (minimized movement)

A

HPO and Oil Immersion Objective

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15
Q

distance of the objective to the stage

A

Working distance

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16
Q

TRUE OR FALSE

The relationship between magnification and the working distance is inversely proportional

A

TRUE

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17
Q

Mechanical Parts

A

Base
Arm
Nose Piece
Fine Adjustment
Coarse Adjustment

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18
Q

Magnifying Parts

A

Ocular
Objectives

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19
Q

Number that expresses the ability of a lens to receive fine detail in an object being observed.

Measure of the resolving power of an objective

A

NUMERICAL APERTURE

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20
Q

Numerical aperture of OIO

A

1.25

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21
Q

It’s NA is 0.25

A

Low Power Objective

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22
Q

It’s Magnification is 4x and has an NA of

A

Scanning; 0.1

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23
Q

HPO magnification and NA

A

40X and 0.65

24
Q

COLOR BANDS

SCANNING
LPO
HPO
OIO

A

SCANNING - Red
LPO - Yellow
HPO - Blue
OIO - White

25
Q

TRUE OR FALSE

MAGNIFICATION IS INVERSELY PROPORTIONAL TO THE NUMERICAL APERTURE

A

FALSE

26
Q

Inversely proportional to numerical aperture; ACCEPTABLE SHARPNESS

A

DEPTH OF FOCUS

27
Q

Inversely proportional to numerical aperture, but directly proportional to the depth of field;

A

RESOLUTION

28
Q

Inversely proportional to numerical aperture; Scope

A

Depth of Field

29
Q

constant image even if the objective was changed

A

PARFOCAL

30
Q

houses the optical parts in the upper part of the microscope

A

HEAD/BODY

31
Q

supports the microscope and houses the illuminator

A

BASE

32
Q

connects to the base and supports the microscope head. It is also used to carry the microscope

A

ARM

33
Q

what you look through at the top of the microscope

A

EYEPIECE

34
Q

holds the eyepieces in place above the objective lens

A

EYEPIECE TUBE

35
Q

primary optical lenses on a microscope; ranging from 4x to 100x

A

OBJECTIVE LENSES

36
Q

Houses the objectives; used to select the objective

A

Nosepiece

37
Q

where the specimen to be viewed is placed; used when working at higher magnifications where delicate movements of the specimen slide are required.

A

STAGE

38
Q

when there is no mechanical stage

A

STAGE CLIPS

39
Q

hole in the stage through which the base (transmitted) light reaches the stage

A

APERTURE

40
Q

used to collect and focus the light from the illuminator on to the specimen

A

CONDENSER

41
Q

controls the amount of light reaching the specimen

A

IRIS DIAPHRAGM

42
Q

moves the condenser up or down to control the lighting focus on the specimen

A

CONDENSER FOCUS KNOBS

43
Q

optical constant of the lens

A

FOCAL LENGTH (mm)

44
Q

distance from the center of the lens to the point where parallel rays entering the lens are brought to a focus

A

FOCAL LENGTH (mm)

45
Q

free space between the specimen surface and the objective

A

WORKING DISTANCE (mm)

46
Q

Measure of the resolving power of an objective

A

NUMERICAL APERTURE

47
Q

the property to recognize features of a specimen to each other as separate and distinct

A

RESOLVING POWER OF AN OBJECTIVE

48
Q

The objectives are optically and mechanically designed so that the distance between the specimen and the aerial image is always constant

A

PARFOCAL

49
Q

It is used in refocusing and resolve the sharpness of the image after changing from one objective to another

A

FINE FOCUS KNOB

50
Q

TRUE OF FALSE:

Place the microscope close to the edge of the table.

A

TRUE

51
Q

It is used to lower the body tube until the 4X or 16mm objective reaches the downward stop.

A

COARSE FOCUS KNOB

52
Q

The circular area seen in the eyepiece

A

FIELD OF VISION

53
Q

It is used for initial focusing and viewing.

A

SCANNING OBJECTIVE

54
Q

TRUE OR FALSE

The higher the magnification, the lower the depth of focus.

A

TRUE

55
Q

TRUE OR FALSE:

The higher the power of objective, the lesser is the area of the specimen surface included in the field of vision.

A

TRUE

56
Q

Thickness of the layer within the boundaries of which all points appear to be sharply in focus

A

Depth of Focus

57
Q

TRUE OF FALSE:

The depth of focus is inversely proportional to the square of the numerical aperture.

A

TRUE

MICROBIOLOGY LAB (3 decks)

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