aseptic technique Flashcards
Microorganisms need to be in the form of ————————–______________ in order for them to be characterized and identified.
pure culture
Microorganisms grow in ————— medium.
sterile nutrient-containing medium
We must have a method of transferring growing organisms (also called ———–) from a pure culture to a sterile medium without introducing any unwanted outside contaminants.
inoculum
Techniques necessary when handling organisms to maintain aseptic conditions
ASEPTIC TECHNIQUES
Performing lab work that greatly reduces the risk of contamination
ASEPTIC TECHNIQUE
Disinfect your working area with the ———–.
10% Lysol or disinfectant
Necessary lab materials used in aseptic technique
bacterial stock culture
growth media
alcohol lamp
transfer tools
proper flame
small blue cone
The inoculated needle and loop needs to be heated until they turn ———–
orange
allow you to obtain single isolated bacterial colonies originating from a single bacterium or arrangement of bacteria
STREAKING PATTERNS
It represents a single type of microorganism that originated from a single cell
colony
Pond bacteria contains ——- bacteria/mL
10^5 to 10^6
Soil contain —— bacteria/mL
more than 10^8
E. coli contain ———–bacteria/mL
at least 10^7
method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample
SPREAD PLATING
method applied for microbial testing of foods or any other samples or to isolate and identify variety of microbial flora
SPREAD PLATING
USED FOR COUNTING THE NUMBER OF COLONY-FORMING BACTERIA PRESENT IN LIQUID SPECIMEN
POUR PLATING
Fixed amount of inoculum (1ml) from a broth/sample is placed in the center of sterile petri dish
Pour plating
molten cool agar
approximate 15mL
used to determine the number of microbes/mL
pour plating
method used to obtain pure cultures
STREAK PLATING
