Lab 2

Question 1

micropipettes

Answer 1

accurately measure volumes 1 mL to .001 mL

Question 2

spectrophotometer

Answer 2

measures solution turbidity at different wavelengths

Question 3

vernier calipers

Answer 3

measure length, width, depth to 1/100th cm

Question 4

microcentrifuge

Answer 4

spin volumes < 1.5 mL in microtubes

Question 5

compound microscope

Answer 5

view specimens .2-1000 microns in size

Question 6

Learning objectives

Answer 6

• > Demonstrate the ability to use the proper tool(s) to measure turbidity or concentration, hydrogen ion concentration, mass, size of an object or organism to 0.01 cm, volume of a liquid, separate mixtures based on density, magnify and view objects or organisms for structural details or to measure other parameters in BIO 204.
• > Demonstrate the ability to make solutions from solid or stock either by % w/v, % v/v, by parts (ppt, ppm, etc.), molarity, X, or other concentrations normally used in biology laboratories, or state in writing the procedure for making such a solution.
• > Be able to use information from an applied SALI to troubleshoot a tool. For example, if the Spec absorbance falls below “0,” what adjustments might you make to the spectrophotometer.

Question 7

The question for this lab

Answer 7

Is the rate of filter feeding by blue mussels the same based on weight or based on the number of the mussels?

Question 8

what was our test organism

Answer 8

the blue mussel Mytilus edulis

Question 9

What can we measure

Answer 9

We can measure the feeding rate of mussels in two ways: (1) the rate of sea water clearing, and (2) using the length of the mussels’ released pseudofeces. We will measure water clarity using an instrument called a spectrophotometer (Spec) that measures the turbidity (cloudiness) of water.