Lab Flashcards
What blood components are disintegrated when hemolysis occurs?
Red blood cells
If a patient experiences diarrhea due to Trichuris vulpis, when should the veterinary technician advise a follow-up fecal examination and deworming?
3 months
What anticoagulant is the most effective preservative for use in blood glucose testing?
Sodium Fluoride
A 10 year old female poodle has just finished a 2 week course of antibiotics for a urinary tract infection. Which urine collection method is preferred for follow-up testing in this patient?
Cystocentesis
Which fecal floatation solution is preferred when testing for the presence of Giardia?
Zinc Sulfate
Which immunoglobulin is present in the greatest concentration in the bloodstream?
IgG
which immunoglobulin is the first antibody made to fight new infection, mainly found in blood and lymph.
IgM
what immunoglobulin is 75% of serum antibodies, found in all body fluids, help against bacteria and viruses.
IgG
what immunoglobulin is mainly found on mucous membranes.
IgA
what immunoglobulin has small amounts in blood, MYSTERIOUS IMMUNOGLOBULIN.
IgD
Given a PCV of 42%, what is the estimated hemoglobin value?
14g/dl
how do you find hemoglobin?
Hemoglobin = PCV/3
What would likely be seen on a urinalysis and CBC of a patient with significant dehydration but that is otherwise healthy?
Increased urine SG and increased PCV
Which erythrocyte abnormality is seen in dogs with lead poisoning?
Basophilic Stippling
Prolonged corticosteroid therapy in dogs is known to cause an increase in serum levels of which enzyme?
Alkaline Phosphatase
What laboratory test evaluates kidney function and is a breakdown product of protein?
BUN
what is a breakdown product of muscle?
creatine
Hyaline casts:
Are clear and colorless
In what species is ketonuria most commonly found?
Bovine
A left shift refers to increased numbers of?
Immature neutrophils
What is the component of urinalysis?
Specific gravity
What is the noncellular portion of coagulated whole blood called after it has been centrifuged?
Serum
What media is selective for gram-negative bacteria?
MacConkey
Translocation can be defined as?
The breakage of 2 chromosomes, resulting in an abnormal arrangement.
An MCV value above the normal reference range suggests?
Macrocytosis
A variation in erythrocyte size is known as?
Anisocytosis
elevated hematocrit
Polycythemia
variation in cell shape
Poikilocytosis
increase in amount of RBCs
Erythrocytosis
Decreased total protein can suggest all the following except?
Dehydration, Renal disease, Overhydration, GI losses.
Dehydration
Pollakiuria is?
Urinate frequently, small volume voided
What is the primary function of fibrinogen?
Hemostasis
Hemostasis is?
clotting
An ammonium biurate crystal is sometimes described as?
Looking like a thorny apple
What part of the urinalysis is measured by a refractometer?
Protein
Calcium oxalate crystals are?
Small squares that contain an X
A common finding on a stained blood smear from an animal with autoimmune hemolytic anemia is?
Spherocytosis
What species has Kurloff cells?
Guinea Pigs
Females have what sex chromosome designations?
XX
The most common uroliths found in feline and canine urine are?
Struvite
Signs of immune-mediated thrombocytopenia include all of these except?
Petechiae, Ecchymoses, Thrombocytosis, Anemia
Thrombocytosis
pinpoint hemorrhage is?
Petechiae
area of bruising
Ecchymoses
increase platelets
Thrombocytosis
decreased RBC
Anemia
An example of specific immunity would be?
IgM antibodies to toxoplasmosis
Which of the following is an example of a selective medium?
MacConkey, Mueller-Hinton, Trypticase soy agar with 5% sheep blood, Trypticase soy agar.
MacConkey
What term is used to describe the ability of a test to determine true positives?
Sensitivity
Which immunoglobulin is the first antibody produced in a primary immune response?
IgM
Immunity that is generated by an animal’s immune system following exposure to a foreign antigen is referred to as?
active immunity
The test that uses an enzyme that causes the conversion of hydrogen peroxide to hydrogen and oxygen what a bacterial colony is placed in the hydrogen peroxide is?
catalase
A positive catalase test will be indicated by the presence of?
bubble
The microscopic morphology of Streptococcus is described as?
coccus is chains
Which of the following does not kill endospores?
Autoclaving, Incineration, Hot-air sterilization, Pasteurization
Pasteurization
What bacterium is pleomorphic?
Corynebacterium
In the gram stain procedure, the mordant is?
Iodine
Most bacteria grow best at pH?
7
On a blood agar plate, an area of complete hemolysis is classified as?
Beta
The humoral immune system involves?
B cells
Centrifuging blood that has been collected in a plain (red-top) tube and allowed to clot
Serum
A test done to help diagnose hyperthyroidism is
T4
Total protein levels are _______ in a dehydrated animal.
increased
It is useful to measure electrolyte values when an animal has
fluid loss
When evaluating the liver of dogs and cats, AST should be evaluated in conjunction with
ALT
what percentage of the glomeruli in both kidneys must be nonfunctional before serum mistry changes indicate renal disease?
70%
Kidney disease results in accumulation of metabolic waste in the blood, a condition known as
Azotemia
The anticoagulant used to preserve blood glucose levels for blood chemistry analysis is
sodium fluoride
When plasma is required for blood chemistry analysis, the anticoagulant of choice is
EDTA
measurement of blood levels or amylase and lipase is used to evaluate function of the
pancreas
Stress and epinephrine release in cats may cause an increase in
glucose
Which of the following substances is a by-product of muscle metabolism, produced at a constant rate and filtered out by the renal glomeruli?
Creatine
Nonrenal causes of increased levels of urea might include
The amount of protein ingested
A box of reagents for blood chemistry analysis labeled for storage at 8 degrees Celsius should be stored
in the fridge
What gland is the most active producer of corticosteroids?
Adrenal glands
The main function of bicarbonate is to
Maintain balanced body pH levels
Icteric plasma is what color?
Yellow
Bile acids aid in the digestion of
fats
Amylase acts to break down
starches and glycogen
What is the noncellular portion of anticoagulated whole blood called after it h centrifuged?
Plasma
what colour tube does plasma go in?
PTT
what tube does serum go in?
RTT
What RBC morphology is commonly found in horses?
Rouleaux formation
Anisocytosis is defined as
variation of size among the same cell
Polychromasia in an immature RBC will appear as a _______ on a blood smear stained with new methylene blue stain
reticulocytes
The phagocytes the respond to an infection are?
Neutrophils
An MCV value below the normal reference range suggests?
Microcytosis
Denatured hemoglobin contained in RBCs is?
Heinz bodies
Small nuclear fragments are?
Howell Jolly bodies
variation in cell size is?
Anistocytosis
an elevated hematocrit is most commonly associated with?
dehydration
the anticoagulant of choice for avian and reptile hematology is?
Lithium heparin
on a CBC all of the following findings can be expected in a patients with an infection, except?
Narrow buddy coat
plasma is composed of?
90% water and 10% dissolved substances
what group of animals would normally expect to find nucleated RBCs?
Parrot, turkey, iguana
macrocytic anemia suggests?
bone marrow pathology
phagocytes are a type of?
white blood cell
what RBC indices indicates cell size?
MCV
what pair is both agranulocytes?
Lymphocytes and monocytes
the preferred sample for most blood chemistry tests is?
serum
the leukocytes most commonly associated with parasitic and allergic conditions is the?
eosinophil
what is the buggy coat found on a refractometer?
not seen
what anticoagulant is the first choice for coagulation studies?
sodium citrate
spherocytes may be seen on a blood smear from a dog suffering from?
IMHA
what blood cell can have vacuoles in its cytoplasm when seen in peripheral blood from a healthy animal?
lymphocytes
what is least likely to cause vaccine failure?
administration during anesthesia
serology is the branch of science involved with detection of?
antibodies or antigens
adaptive of specific immunity includes?
the response of the defenses of the body to a specific substance
when T cells are processed in the ____ they develop specific antigen receptors on their cell membranes
Thymus
ELISA is an acronym for?
Enzyme linked immunosorbent assay
the humoral immune system involves?
B cells
the type of immunity that is the function of B cells that transform into plasma cells and produce protective proteins called antibodies agains antigens is
humoral
T killer cells function to?
recognize cancer cells as abnormal cells and eliminate them