Lab 10 Endospore stain

Question 1

What is a Bacterial endospore?

Answer 1

bacteria that have extremely durable over-weathering structures that resist harsh environments and remain viable for millions of years

Question 2

What is a spore

Answer 2

• refers to a single cell that is in a state of semi dormancy
• under the right conditions, the spore will divide and grow into a later and more active form of the organism
• structurally distinct from sex cells; sperm, egg cells

Question 3

What are the two similarities bacterial spores have with plant and mold spores?

Answer 3

-haploid and dormant

Question 4

Differences between bacterial spores vs plants and mold spores?

Answer 4

• All bacteria are haploid; bacteria reproduce only asexually ( binary fission); there is no diploid part of their lifecycle, unlike for plants and molds.
• Bacterial spires do no grow into multicellular structures, since bacterial are unicellular organisms

Question 5

Define dormancy

Answer 5

no detectable metabolic activity whatsoever in a bacterial endospore

Question 6

Types of bacterial endospores

Answer 6

• clostridum
• bacillus
• rhizopus

Question 7

What is a vegetative cell?

Answer 7

• metabolically active cells

- cells that are taking in nutrients, building biological molecules, releasing water, and dividing (reproducing)

Question 8

What is sporulation?

Answer 8

• similar to binary fission
• A copy of a bacterial chromosome is made. The cell then divides, creating 2 cells.
• one of the cells in smaller than the other; the smaller cell is destined to become an endospore, later engulfed by the larger cell.
• chemical composition changes; nearly all water is removed and accumulates organic acids,
• sporulating cell develops thick coat

Question 9

-Define dessicated

Answer 9

lacking water

Question 10

What is germination?

Answer 10

• when a bacterial endospore takes on water, expels its organic acid preservative, and converts into a metabolically active (vegetative) cell

Question 10

What is germination?

Answer 10

• when a bacterial endospore takes on water, expels its organic acid preservative, and converts into a metabolically active (vegetative) cell

Question 11

What are bacterial endospores resistant to?

Answer 11

heat
radiation
disinfectants