Biotyping Based on Fermentation Lab 16 Flashcards
What are the reactants and products of Glycolysis
Reactant: 1 glucose
products:2 pyruvate
Describe the characteristics of Glycolysis
- anaerobic:does not need 02
- the energy released allows the cell to make 2 ATP
What is vital for cell to continue doing Gylcolysis
-2 NAD+ is vital
no NAD+=no ATP yielded
What does fermentation metabolism do?
- regenerates NAD+ so that glycolysis can continue without O2
- converts NADH back into NAD+ by transferring electrons onto an organic molecule
What are the products of fermentation metabolism? What can they be used for?
-acids, gases, alcohols; can be sometimes used as building blocks, but are usually just waste products
What is the relationship between glycolysis and fermentation metabolism?
Fermentation metabolism regenerates NAD+ so that glycolysis can continue without O2
Explain what happens in Aerobic cell respiration that fermentation cannot do
- In aerobic cell resp, energy can be extracted from NADH and pyruvate
- pyruvate is further oxidized CO2, creating more electron carriers NADH and FADH2
What does the electron transport chain do?
- oxidizes electron carriers back to NAD+ and FAD, allowing cell respiration reactions and glycolysis to continue
- transfers electrons to O2 and becomes H20
What happens as electrons move though the electron transport chain, and type of energy is produced?
- Hydrogen ions are pumped out of the bacterial cell
- this creates a high concentration of protons/positive charges outside of the cell which creates the proton motive force.
What is mineral oil used for in the OF glucose test?
-Mineral oil prevents the addition of O2
What does the OF glucose test for?
-is bacterial ferments glucose and peptone
What does the OF glucose test for?
-if bacterial specimen ferments glucose and peptone
What do the results of the OF glucose test indicate?
yellow=bacteria fermented the glucose into acids/cell resp
blue=deamination of amino acids (peptone),ammonia raises ph
What does the methyl red test for?
-tests for mixed acid fermentation; lactate, acetate, formate
How does the methyl red test indicate for a positive test for mixed acid? How long does it take to see results?
- turns red
- can see results immediately
What does the VP test for?
-butanediol fermentation
How does a VP test indicate a positive result of butanediol fermentation? What reacts with the VP reagents for this to be produced?
-produces a red color; acetoin is reacted with
What does the citrate utilization test for?
- if an organism can import citrate into their cells using citrate permease
- these cells can use citrate as their sole course of energy/building blocks
What do the results in a citrate utilization test show?
- Blue= positive
- green= negative
