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Anatomy 2 Lab > Lab 1: Blood Physiology > Flashcards

Lab 1: Blood Physiology Flashcards

1
Q

hematocrit (Hct) % formula

A

= (height of red cells (mm)/height of red cells and plasma (mm)) x 100

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2
Q

Hb standard for 6.4 g

A

82%

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3
Q

Hb standard for 11.2 g

A

69%

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4
Q

Hb standard for 19.9 g

A

45%

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5
Q

Blood Hematocrit (Hct) experiment

A

for both males and females it is slightly higher than the “normal” hematocrit %

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6
Q

Hct % for males

A

average: 46%, range: 43-49%

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7
Q

Hct % for females

A

average: 41%, range: 36-45%

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8
Q

how many mL of O2 does 1 g of Hb can carry?

A

1.34 mL

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9
Q

each 100 mL of blood has 15 g of Hb, which can carry how many mL of O2?

A

20 mL

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10
Q

anemia

A

results from an abnormal decrease in the # of erythrocytes, tissues become oxygen starved

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11
Q

hematocrit

A

percent volume of whole blood that is occupied by red blood cells

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12
Q

severe anemia hematocrit %

A

15%

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13
Q

polycythemia hematocrit %

A

70%

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14
Q

up to 34% of the RBC by weight is

A

hemoglobin

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15
Q

concentrations of hemoglobin male

A

average: 15.4g/100mL blood
range: 13.6-17.2

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16
Q

concentrations of hemoglobin female

A

average: 13.3g/100mL blood
range: 11.5-15.0

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17
Q

anemic

A

less than 10g/100mL of blood

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18
Q

cyanmethemoglobin test

A

involves the reaction of hemoglobin with a reagent solution containing potassium cyanide (KCN) to form cyanmethemoglobin

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19
Q

concentration of cyanmethemoglobin

A

determined by comparing the amount of light it can absorb compared to the amount of light absorbed by known concentrations of hemoglobin

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20
Q

each g of hemoglobin carries how many mL of blood in rats?

A

70 mL per kg

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21
Q

total blood Hb (g) formula

A

= (blood volume mL blood) x (hemoglobin concentration g Hb/mL blood)
hemoglobin concentration g Hb/mL blood pe

22
Q

total O2 carrying capacity formula

A

= (total g Hb) x (1.34 mL O2/Hb)

23
Q

hemoglobin concentration g Hb/mL blood per ONE ml of blood

A

for example, covert 25 Hb /100 mL to 0.25 Hb/1mL

24
Q

blood volume in males

A

79 mL blood per kg

25
Q

blood volume in females

A

65 mL blood per kg

26
Q

average total leukocyte count

A

7,500/mm^2

27
Q

neutrophilic leukopenia

A

protozoan infections, malnutrition, aplastic anemia

28
Q

neutrophilic leukocytosis

A

strenuous exercise, rheumatic fever, severe burns

29
Q

lymphocytosis

A

mumps, German measles, whooping cough

30
Q

eosinophilia

A

scarlet fever, parasitic infections, allergic reaction

31
Q

monocytosis

A

chronic diseases, such as tuberculosis and leukemia

32
Q

lymphocytopenia

A

administration of glucocorticoid drugs

33
Q

two classes of leukocytes (WBC)

A
  1. granulocytes (Polymorphonuclear leukocytes)
  2. agranulocytes (mononuclear leukocytes)
34
Q

granulocytes

A
  1. neutrophils
  2. eosinophils
  3. basophils
35
Q

agranulocytes

A
  1. small lymphocytes
  2. large lymphocytes
  3. monocytes
36
Q

neutrophils

A

small pink cytoplasmic granules
purple nucleus
multi-lobed (3 or more) nucleus

37
Q

eosinophils

A

coarse red-orange cytoplasmic granules
blue-purple nucleus
bilobed nucleus

38
Q

basophils

A

black nucleus
large, deep-blue or reddish-purple cytoplasmic granules
bilobed nucleus

39
Q

small lymphocytes

A

light blue cytoplasm (nongranular)
dark purple nucleus
very large, spherical nucleus surrounded by a thin cytoplasm

40
Q

large lymphocytes

A

light blue cytoplasm (nongranular)
dark purple nucleus
large oval, intended nucleus

41
Q

monocytes

A

light blue-gray cytoplasm (nongranular)
blue or purple nucleus
large, deeply indented nucleus (horseshoe-shaped)

42
Q

how many leukocytes do you need to count to perform leukocyte count procedure?

A

100 leukocytes

43
Q

blood hematocrit experiment

A

-use rat blood
-use watch glass
-use heparinized tube
-use micro-hematocrit centrifuge
-apply Hct % formula

44
Q

Hemoglobin determination experiment: % transmittance (results)

45
Q

Hemoglobin determination experiment

A

-place a cyanmethemoglobin reagent in a cuvette
-add blood
-cover the cuvette with parafilm
-obtain % transmittance
-Spec 20 is used

46
Q

identification of WBCs and the differential leukocyte count experiment

A

-obtain a blood smear slide
-use a microscope
-count leukocytes
-follow a pattern as you are counting them (left to right, up and down)

47
Q

red blood cells males

A

5.4+-0.8 million per mm^3

48
Q

red blood cells females

A

4.8+-0.8 million per mm^3

49
Q

white blood cells males

A

7-9000 per mm^3

50
Q

white blood cells females

A

5-7000 per mm^3

51
Q

platelets

A

150,000 to 400,000 per mm^3
average: 300,000 per mm^3

Anatomy 2 Lab (2 decks)

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