L6 AlphaFold Flashcards
What is AlphaFold?
AI platform which performs prediction of protein structures using aa sequence
What does the structure of proteins determine?
function
What do 34% of all FDA approved drugs target?
G coupled protein receptors
What structures are confidently predicted & what are not?
Alpha helices & beta sheets are confidently predicted - loops not as much
What happens if something has a PI in the basic range?
it can be more positively charged than negatively charged
What structure is the most conserved part of the protein?
Transmembrane domains
What does structural biology look at?
Proteins & nucleic acids at molecular level
What does structural biology unlock?
Secrets of structure, function & interactions
What is macromolecular crystallography?
Mature technique that permits structures to be obtained rapidly after sample crystallization
What can macromolecular crystallography results be complemented by?
can be complemented by small angle solution x ray scattering
What has NMR be particularly useful for?
To examine macromolecular dynamics in solution & membrane proteins in solid state
What do Cryo electron microscopy permit?
Near atomic resolution on macromolecular complexes
When was AlphaFold launched?
2021 by Google DeepMind & EMBL-EBI
What can AlphaFold 3 generate?
Accurate strcutures containing DNA, RNA, ligands, ions & can also model chemical modifications for proteins & nucleic acids
