L5 Regulating DNA Transcription

Question 1

What regulates gene transcription?

Answer 1

The binding of gene activator proteins to gene promotors
As well as their interaction with RNA polymerase

(Their presence or their ability to enter the nucleus)

Question 2

Some properties of gene activator proteins?

Answer 2

Modular structure
Work synergistically
can exist downstream of TATA

Question 3

When are chromatin loosely packed?

Answer 3

Interphase

Question 4

Gene activator proteins often work…

Answer 4

SYNGERGISTICALLY

Question 5

Other than binding onto the DNA, how can GEne activator proteins affect transcription?

Answer 5

Gene activator proteins can have a role in unwinding chromatin

It can:
Remodel nucleosomes 
Histone removal 
Histone replacement
Specific pattern of Histone modification

Question 6

What are corepressor and coactivators?

Answer 6

These often assemble with gene regulatory proteins and affects transcription

Question 7

What is combinatorial control?

Answer 7

The hyper initiating probability of transcription when there are multiple regulatory factors

Question 8

What is a method that can be used to identify which gene the enhancer region enhances?

Answer 8

Recombinant DNA technology

Placing a section of the Dna With gene promotors in front of artificially made gene, like lac z, to see which region will express.