L4.2 Plasmids

Question 1

Methods of plasmid transfer

Answer 1

Conjugation
Transduction
Transformation

Question 2

What happens in conjugation

Answer 2

the donor F+ cell form a bridge or pilus to attache to the recipient F- cell

Question 3

Are all plasmids conjugative?

Answer 3

No
conjugative plasmids contains TRA gene (trasnfer gene)

Question 4

Direct contact, transfers DNA strand, attachment of pilus/pili

Answer 4

Conjugation

Question 5

Happens between virus and bacteria. Incorporates the bacterial chromosomal DNA into a virus particle. Virus will transfer it to a new DNA of recipient.

Answer 5

Transduction

Question 6

Involves a dead donor cell leaving a fragment of DNA

Answer 6

Transformation

Question 7

Trait of the plasmid
ColE1

Answer 7

bacteriocin kills E.coli

Question 8

Tol

Answer 8

degradation of toluene and benzoic acid

Question 9

Ti

Answer 9

Tumor initiation in plants

Question 10

pJP4

Answer 10

2,4-D (dichlorophenoxyacetic acid) degradation

Question 11

pSym

Answer 11

nodulation on roots of legume

Question 12

SCP1

Answer 12

antibiotic methylenomycin biosynthesis

Question 13

RK2

Answer 13

resistance to ampicillin, tetracycline and kanamycin

Question 14

Types of bacterial plasmids
Fertility (F) plasmids

Answer 14

• has tra genes, conjudation, has pilus for cell-cell interaction

Question 15

Resistance (R) plasmids

Answer 15

antibiotic resistant gene, R-factors, large

Question 16

COL-plasmids

Answer 16

codes for colicines a type of bacteriocin that can kill bacterial cells

Question 17

Degradative plasmids

Answer 17

digestion of chemicals, toulene

Question 18

Virulence plasmids

Answer 18

virulence factors

Question 19

Enumerate all the types of bacterial plasmids

Answer 19

Fertility
Resistance
COL
Degradative
Virulence

Question 20

How is conformation of plasmid DNA seen?

Answer 20

through agarose gel and relative migration

Question 21

Observed for native DNA conformation in vivo, extra twist, migrate faster
- desired conformation for isolating plasmid DNA

Answer 21

Supercoiled or covalently closed circular

Question 22

DNA is intact but no supercoils

Answer 22

Relaxed circular

Question 23

occurs due to excessive alkaline lysis, compacted plasmid form
single stranded, the fastest to migrate

Answer 23

supercoiled denatured

Question 24

topoisomerases cut a portion of the supercoiled DNA to gain access for replication.
slowest conformation in the agarose gel

Answer 24

Nicked open circular

Question 25

cut at both ends in one site only

Answer 25

linearized

Question 26

Categorize plasmid conformation based on migration

Answer 26

1. Supercoiled Denatured
2. Supercoiled
3. Relaxed circular
4. Linear
5. Nicked open circular