L4. Protein Folding and Stability

Question 1

4.1 Protein Folding
Equilibrium

Question 2

4.1 Protein Folding
Thermodynamics

Question 3

4.1 Protein Folding
Problem

Answer 3

Ask 3 questions
- what route do polypeptides take to undergo folding
- how does the primary sequence dictate protein structure
-can we design a program to predict the fastest route to get to the most stable 3D structure

Question 4

4.2 Protein Stability 2 forces

Answer 4

Hydrophoci collapsr
H bonding

Question 5

4.3 Disorder
Name of common disorder structures

Answer 5

Molten Globule

Question 6

4.3 Disorder
Molten Globule

Answer 6

More water penetration and greater dynamics
Some tertiary contacts are present and water mediate interactions are present

Question 7

4.3 Disorder
Function Paradigm

Question 8

4.3 Disorder
Intrinsecally Disordered Protein

Question 9

4.3 Experiments
Name all

Answer 9

• Circular Dichroism (CD) Spectroscopy
• Thermal Shift Assay
• Chemical Denaturation

Question 10

4.3 Experiments
CD Spectroscopy

Question 11

4.3 Experiments
Thermal Shift Assay

Question 12

4.3 Experiments
Chemical Denaturation

Question 13

AlphaFold

Answer 13

Is a computational prgram that utilizes homology-modelling, takes struxtures deposited into the PDB to predit protein structures from the primary sequence.