L4- Glycoprotein therapeutics for emerging viruses

Question 1

Discuss the glycosylation of proteins and the function of glycans. Why is studying them important in terms of viruses?

Answer 1

Slide 4-9

Question 2

Discuss the structure of human glycans.

Answer 2

Slide 10-18

Question 3

How can amino acid sequence define glycan attachment?

Answer 3

Slide 19-20

Question 4

What are the stages of N-linked glycan attachment and processing?

Answer 4

Slide 21-27

Question 5

What determines the composition of a fully processed glycan?

Answer 5

Slide 28

Question 6

Discuss O-linked glycosylation.

Answer 6

Slide 29

Question 7

How do glycoproteins help Ebola survive?

Answer 7

Slide 30

Question 8

Discuss the diversity of HIV and the issue with this.

Answer 8

Slide 32-33

Question 9

How does the glycan shield of HIV affect vaccine design and how can bNAbs overcome this?

Answer 9

Slide 34-36

Question 10

Discuss the mannose patch of HIV gp120.

Answer 10

Slide 37-41

Question 11

Discuss the development of soluble Env trimers.

Answer 11

Slide 42-66

Question 12

Discuss the mannose patch of Lassa virus.

Answer 12

Slide 67-70

Question 13

Discuss the journey to build the full structure of the SARS-Cov-2 spike.

Answer 13

Slide 71-82