L3 - Receptors 2 Flashcards
what are some examples of G-coupled receptors
muscarinic acetylcholine receptors
adreno receptor
dopamine receptors
opiate receptors
what are the general parts of a G protein receptor
n-terminus binding domain
Intracellular C-terminus
7 transmembrane domains (M1-M7)
A g-protein coupling domain
What are the steps to GPCR signaling
bind N-terminus
GTP replaces GDP on a-subunit
B, y leaves a-subunit
target activates
GTP to GDP+Pi by GTPase
a-subunit rebinds with B, y
in the heart muscles what affects adenylatt cyclase (AC) enzyme
cardiac muscarinic acetylcholine (Gi), B-adrenoreceptors (Gs)
why is AC a target for G-proteins
AC makes cAMP which GCPRs regulate
what does cAMP bind to and regualte
other enzymes
transporters
ion channels
smooth muscle contractile protein
voltage-gated Ca2+ heart cell channels
What is an example of G-protein receptors
multifunctional B-adrenoreceptor
what do the B-adrenoreceptors do
regulate heart contractions
what is it used for in the liver
used for glycogen metabolism
what is the agonist for the B-adrenoreceptors
adrenaline/epinephrine
what is phospholipase C
a target for G-protein receptors
what are the 2 messengers of phospholipase C
IP3: adding free Ca2+ by releasing it from some parts
DAG: controls protein function by phosphorylation (protein kinase)
ion-channels are also targets of G-protein what do they transport
Ca2+, K+ and Na+ channels
