L3 Genetic Techniques

Question 1

Which genetic testing method amplifies small regions of DNA to allow them to be detected or measured?

Answer 1

Polymerase chain reaction (PCR)

Analysis is usually done by electrophoresis

Question 2

What general term refers to determining the order of nucleotide bases that make up DNA?

Answer 2

DNA sequencing

Question 3

Western blotting is used to detect what?

Answer 3

Protein

Remember: SNOW DROP (SNW DRP)

Southern = DNA
Northern = RNA
Western = Protein

Question 4

Southern blotting is used to detect what?

Answer 4

DNA

Remember: SNOW DROP (SNW DRP)

Southern = DNA
Northern = RNA
Western = Protein

Question 5

Northern blotting is used to detect what?

Answer 5

RNA

Remember: SNOW DROP (SNW DRP)

Southern = DNA
Northern = RNA
Western = Protein

Question 6

What blotting method is used to detect fragile X syndrome?

Answer 6

Southern blotting - detects the altered DNA

Question 7

Connexin 26 (GJB2) D35G is the most common inherited cause of __?__

Answer 7

deafness

Question 8

Cystic fibrosis mutation (__?__) can be detected by simple PCR assay.

Answer 8

F508del

Question 9

Which genetic testing method can look at single genes or just a few genes at a time and relies on a special chemical that marks each DNA nucleotide with a different coloured fluorescent dye.

Answer 9

Sanger sequencing

Question 10

Which genetic testing method involves separating whole chromosomes from the nucleus of a cell on a glass slide and taking pictures?

Answer 10

Karyotyping