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Biochemistry BCC > L28: Recombinant DNA technology II > Flashcards

L28: Recombinant DNA technology II Flashcards

1
Q

What is polymerase

A

an enzyme which brings about the formation of a particular polymer, especially DNA or RNA.

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2
Q

Define Recombinant

A
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3
Q

An enzyme which catalyses the formation of a polymer

A

Polymerase

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4
Q

Allows selective sysnthesis of a gene sequence 100bp -10000bp in length

A

Polymerase chain reaction

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5
Q

Define: Polymerase chain reaction

A
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6
Q

The word _____ is derived from the Greek root poly-, meaning many, and mer,

A

Polymer

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7
Q

Say 5 things you know about Sanger Sequencing

A

1
2
3
4
5

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8
Q

define Sanger sequencing

A
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9
Q
A

Sanger Sequencing.

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10
Q

What is in vitro DNA synthesis in a tube?

A
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11
Q

What happens when you heat DNA pol to 95 degrees?

A

They will nature

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12
Q

What is ssDNA primer?

A
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13
Q

What in dNTPs

A

Deoxy…….

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14
Q

What is DNA polymerase?

A
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15
Q

What is ssDNA template?

A
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16
Q

How is ssDNA obtain?

A

Heat up to 95 degrees

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17
Q

What is Taq Polymerase?

A
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18
Q

What is thermus aquaticus?

A
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19
Q

What is an advantage to thermus aquaticus?

A

Withstands repeated heating and cooling cycles without denaturing

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20
Q

Why is mg2+ an essential cofactor in thermus aquaticus?

A
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21
Q

What reaction requires knowledge of gene sequence required to design ssDNA primers?

A

Polymerase chain reaction

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22
Q

Name 3 uses of Polymerase chain reaction uses

A
  1. Gene cloning: amplification of defined gene sequence
  2. VIral screening - high sensativity, detect virus before showing disease (covid)
  3. Forensics - DNA finger printing, show familial linkage, e,g OJ Simpson (DNA testing)
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23
Q

In PCR, what is primer pair selection?

A
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24
Q

How many primers are needed for dsDNA sysnthesis?

A

2

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25
_____ ______ defines region to be amplified
Primer pair
26
What is a restriction enzyme?
27
Restriction enzymes are not needed in any step of which kind of reaction?
Polymerase chain reaction.
28
What is a DNA oligonucleotide?
29
DNA oligonucleotide
30
What does annealing mean?
31
What is a primer annealing temperature?
32
How many hydrogen bonds in A+T ?
33
How many hydrogen bonds in C+G?
34
ssDNA primer
35
What is the process of PCR?
1.95 degrees - 2. 42-65 degrees - 3. 72 degrees -
36
Define Taq polymerase
37
define DNA synthesis
38
What is DNA synthesis
39
DNA synthesis
40
In PCR, what is linear amplification?
41
What are PCR cycles?
42
What are PCR long v short DNA products?
43
What is gel electrophoresis?
44
What is Sanger DNA sequencing?
45
What are dideoxyribonucleotides?
chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing
46
dideoxyribonucleotides?
47
This was used to obtain the human DNA sequence
Sanger Sequencing
48
What are the applications of Sanger Sequencing?
1. Determine the order of baes from single gene to whole genome. 2.Screens for gene mutations and sequence variants 3. Validate the sequence of a PCR product
49
Define: dideoxyribonucleotides chain terminators
50
what are dideoxyribonucleotides? triphosphates? dNTPs
51
Etymology of dideoxyribonucleotides?
52
what is ddCTP?
53
what is dideoxyATP
54
what is dideoxyGTP
55
what is dideoxyTTP
56
what dieoxy ctp?
57
what is ddATP
58
what is ddGTP
59
what is ddctp(?)
60
What is ddNTP?
61
What is 'gel' in DNA?
62
What is gel electrophoresis?
63
what are fluorescent ddNTP's?
64
what is a flourophore in sanger sequencing?

Biochemistry BCC (7 decks)

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