L221 Diagnostic Teqs Flashcards
Subtraction radiography can detect bone density change of ____
5 percent
Limitations of radiographs
Limited sensitivity
Sensitivity
True positive / (true positive + False negative)
Specificity
True negative / (True negative + False positive)
Probability of test being negative when disease is not present
Sensitivity of bacterial culturing
Low
Why is it difficult to use direct microscopy
Most of the main perio pathogens are non motile so theyre hard to see
Antibody detection with immunofluorescent microscopic assy is used mainly to detct which bacteria
Aa and Pg
Pg Tf and Aa possess a common _________ enzyme that hydrolyzes _________
Pg Tf and Aa possess a common trypsin like enzyme that hydrolyzes BANA
PCR as a method
High sensitivity and specificity
Sources of samples
Saliva GCF Blood serum Blood cells Urine (hypophosphatasia)
GCF components
Host derived enzymes and their inhibitors
Byproducts of tissue breakdown
Inflammatory mediators
Aspartate amino transferase
Released during cell death
Alkaline phosphatase
Membrane bound glycoprotein involved in maintenance of alveolar bone
Beta-glucuronidase
Lysosomal enzyme degrades proteoglycans and ground substance
Elastase
Proteolytic enzyme in lysosomal granules of neutrophils
Extracellular destruction enzymes are associated with
Matrix metalloproteinases
Intracellular destruction enzymes
Aspartate aminotransferase
Alkaline phosphatase
Beta-glucuronidase
Elastase
Intracellular destruction enzyme with high sensitivity and specificity
Beta glucuronidase
Changes in bone can be identified by eye only after _____ bone loss
30-50 percent
Bioplex cytokine assay
Color coded beads to quantify cytokines in tissue
IR spec sensitivity and specificity
High sensitivity
High specificity
Near Infrared spec measures
measures oxygen saturation in tissues 500-600nm
Over expression of which cytokines give genetic susceptibility
IL-1alpha
IL-1beta
Salivary Occult Blood Test use
When thorough periodontal examination is not possible
Bioplex cytokine assay
Color coded beads to quantify cytokines in tissue
IR spec sensitivity and specificity
High sensitivity
High specificity
Near Infrared spec measures
measures oxygen saturation in tissues 500-600nm
Over expression of which cytokines give genetic susceptibility
IL-1alpha
IL-1beta
Salivary Occult Blood Test use
When thorough periodontal examination is not possible
