Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIOCHEM FINALS** > L20&21- Fermenters (#3) > Flashcards

L20&21- Fermenters (#3) Flashcards

1
Q

Carbohydrate digestion generates__?

A

Glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Glucose fermentation generates?

A

Volatile Fatty Acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Methane production allows___ to continue

A

Glycolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

VFAs are used to make ____ aka ( __ )

A

Glucose (gluconeogenesis)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

VFA are used to make ____________ as well (other than glucose)

A

FA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Most forgut fermenters are ________

A

ruminants

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Ruminant stomach is divided into 4 compartments:

A
  1. Rumen
  2. Reticulum
  3. Omasum
  4. Abomasum
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

____ is the largest of the compartments and functions as a fermentation vat

A

Rumen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Ruminants secrete copious amounts of ____ which is rich in _____ and ______ (needed to buffer acids produced during fermentation, ____ (antifoaming agent), and ___ which helps supply nitrogent for microbial AA synthesis)*

A
  • Saliva.. bicarbondate and phosphate
  • Mucus (antifoaming agent)
  • Urea
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Contents are mixed by ____ of the rumen pillars and wall- how many per minute?

A

Contractions- 1-3 contractions per minute

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

During _____, a bolus of fibrous material is formed at the base of the esophagus and regurgitated so that animal can rechew the dietary fibers*

A

Rumination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Fermentation products, VFAs, are absorbed from the rumen via _____

A

Papillae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

______ allows the ruminant to expel the volumes of gas generated during fermentation*

A

Eructation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the significance of papillae?*

A

Increase SA of the rumen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Ruminants are divided into 3 cateogories based on dietary preference- what are they? Also give example animals of each

A
  1. Grazers (cattle, sheep, buffalo, etc)
  2. Browsers (deer, moose, giraffe)
  3. Intermediate feeders (goats, reindeer, elk, etc)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What are the main differences btwn grazers and browsers?*

A

GRAZERS: have a large rumen allowing for maximal fiber digestion and requiring long fermentation times- their diet is HIGH in fiber and LOW in easily digested carbs

BROWSERS: have a less developed rumen, with short fermentation times- their diet is HIGH in more soluble, easily digested carbs and LOWER in fiber

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What are some major differences between grazers and browsers with respect to RUMEN SIZE?** [+/- too]

A

Grazer (+): Very large, needed to accommodate high-fiber diet

Browser (-): Smaller, deit is lower in fiber, higher in soluble carbs which are more rapidly digested and fermented

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What are some major differences between grazers and browsers with respect to RUMEN PILLARS?** [+/- too]

A

Grazer (+): Well-developed musculature needed to mix bulky, fibrous diet

Browser (-): Musculature is less well-developed bc of lower-fiber diet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What are some major differences between grazers and browsers with respect to RUMEN PAPILLAE?** [+/- too]

A

Grazer (-): Less extensive, VFA are produced at a mod pace, so do not need to be rapidly absorbed

Browser (+): Very extensive. Allows for the rapid absorption of VFA resulting from rapidly fermented soluble carbohydrates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What are some major differences between grazers and browsers with respect to OMASUM?** [+/- too]

A

Grazer (+): Well developed. Omasum keeps undigested fiber from entering abomasum

Browser (-): Less developed. Less fibrous diet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What are some major differences between grazers and browsers with respect to SALIVARY GLANDS?** [+/- too]

A

Grazer (-): Moderate (cow prod 100-150 L/day)

Browser (+): Well developed (3 to 4 times the amnt of grazer). Saliva buffers the rapidly produced VFA and glands secrete tannin binding proteins. Copious saliva washes easily digested food directly into abomasum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What are some major differences between grazers and browsers with respect to RAFT OR MAT FORMATION?** [+/- too]

A

Grazer (+): High fiber diet leads to mat formation

Browser (-): Low fiber diet decreases tendency to form mat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What are some major differences between grazers and browsers with respect to FERMENTATION TIME?** [+/- too]

A

Grazer (+): Very long. Long time needed to digest and ferment the high-fiber diet

Browser (-): Shorter time. Low-fiber, high-soluble carb diet, is fermented more rapidly

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What are some major differences between grazers and browsers with respect to BIOHYDROGENATION?** [+/- too]

A

Grazer (+): Extensive hydrogenation bc of long fermentation time and need to dispose of hydrogens generated during fermentation. Fewer PUFA in body fat of ruminant

Browser (-): Less hydrogenation. Higher cc of PUFA are incorp in body fat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

What are some major differences between grazers and browsers with respect to GLUCOSE TRANSPORTERS?** [+/- too]

A

Grazer (-): Fewer glucose transport proteins in SI

Browser (+): Present. Some readily available carbohydrates (RAC) are swept out of the rumen because of the large volume of saliva and are digested and absorbed in the SI

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

What are the 2 major categories of carbs in forage?* Describe

A
  1. Cell contents or solubles carbs- easily digested and make up a larger percentage of the carbs in the diet of the browser
  2. Cell wall components- are more resistant to digestion and make up the majority of the carbs in the forage of grazers
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

_____ is composed of linear chain of glucose with B-1,4 linkages- several chains will aggregate to form microfibrils- what is exclusive about mammalian enzymes with the B-1,4 linkages?

A

Cellulose

- No mammalian enzymes can hydrolyze it

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

____ has a random structure with many diff sugards besides glucose such as xylose, manose, etc

A

Hemicellulose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

____ is a branched polysaccharide rich in galacturonic acid which fnctions as a glue to hold neighboring plant cells together

A

Pectin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

____ is highly insoluble phenolic polymer crosslinked to the hemicellulose and pectins. _____(repeat) adds rigidity to the plant cell walls

A

Lignin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Lignification increase with plant ____ and decreases both _____ and ___ of forage

A
  • Plant age

- Digestibility and palatability of forage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

3 types of rumen microorganism (RMO) are?

A
  1. Bacteria
  2. Protozoa
  3. Fungi
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

____ are the ultimate specialists

A

Bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

What do protozoa engulf? (3 things)

A

Fiber fragments, starch granules and fiber-digesting bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Digestion of the plant cell walls is carrid out by a __________ of several bacterial species- what does this mean?

A

Consortium
- Alone one species would be unable to digest but coming together to form a consortium of several species, they can digest cell wall fiber to glucose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

What is termed a “readily available carb” (RAC) in contracst to cellulose and hemicellulose which are NOT easily digested and their glucose is NOT therefore readily available?

A

Starch

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

What happens ot the glucose obtained from the digestion of carbs from cattle feed?

A

Fermented by RMO in 2 steps:

  • Primary fermentation
  • Secondary fermentation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

What is primary fermentation?

A

Uses glycolysis to convert glucose to pyruvate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

What is secondary fermentation?

A

Converts pyruvate to a variety of VFA mainly (acetate, pripionate, and butyrate and some gases)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

What are the endproducts of rumen fermentation*? (4)

A
  1. VFA (acetate, propionate, and butyrate)*
  2. Gases (CO2, CH4, H2, HS)
  3. Heat of fermentation
  4. Microbial cell mass (princip source of protein for ruminants)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

Fermentation only occurs in absence of ____

A

Oxygen

42
Q

In ____ glycolysis, glucose oxidation results in the formation of ATP, NADH, and pyruvate

A

Aerobic

43
Q

Methane generation provides the means of removing the hydrogen from ______ and allowing a greater total yield of ATP

A

NADH

44
Q

___________ converts formate to methan

A

Methane-prod bacteria aka methanogens

45
Q

Menthane production presents a MAJOR LOSS OF _____ for the ruminant

A

Energy

46
Q

The quantity of celluose and starch in diet influences the proportion of fermentaion endproducts- aka a _____ molar ratio)

A

Acetate to propinoate (A:P)

47
Q

A high-fiber diet prod more ___ than propionate, this ratio is close to the number ____

A

more acetate than propinoate

A:P close to 3

48
Q

A high-grain diet has a A:P ratio closer to ________

A

1

49
Q

Prpinoate prod is inversely related to methane production, therefore a high-fiber diet would be ______ (higher/ lower)

A

Higher

50
Q

Prpinoate prod is inversely related to methane production, therefore a high-grain diet would be ______ (higher/ lower)

A

Lower

51
Q

A:P Molar ratio changes with ___

A

Feed

52
Q

Acute lactic acidosis can occur when pasture-fed cattle are bourght to feedly and _____

A

Abruptly started on a high-grain diet

53
Q

When a pasture-fed ruminant with a primarly fiber-digesting microflora is abruptly given a high-grain diet, the shortage of starch-digesting and sugar-utilizing microflora is overcome by an ___, ___________ lactate-producing bacteria

A

Opportunistic , fast-growing

54
Q

Acute Lactic Acidosis: Why does rumen become acidic? (3)*

A
  1. Lactate is not as rapidly absorbed from rumen as the VFA (acetate, prop, buty)
  2. Lactate is stronger acid than VFA (pKa of lactate is 3 versus pKa of 4.8 for acetate)
  3. Saliva prod decreases on a low-forage, high-grain, diet depriving rumen of a much needed buffer
55
Q

Acute Lactic Acidosis: Why does lower pH precipitate a downward spiral?

A
  1. Growth rate of other RMO slows
  2. The rumen, unlike abomasum has no mucus to protect itself from this increasingly acidic environment
  3. Rumen papillae slough off creating breaks in surface
56
Q

Acute Lactic Acidosis: How can we treat this?

A
  • Gradual shift in diet instead

- Acid rumen can be treated by giving sodium bicarbonate

57
Q

VFA is used as a fuel for muscle precursors for ____ and for FA synthesis

A

Gluconeogenesis

58
Q

VFAs are either ___ or ___

A

ketogenic or glucogenic

59
Q

Butyrate is cleaved by ____ to form 2 acetyl-coAs

A

B-oxid

60
Q

What are the MITV amino acids used for converting Propinoyl coA to succinyl coA using methylmalonyl Coa?

A

Methinine
Isoleucine
Threonine
Valine

61
Q

Pathway of prpinoyl to succinyl coA production requires vitamins (3)?

A
  1. Pantothenic acid –> Coeznme A moieties
  2. Biotin –> propinyl coA carboxylase
  3. Bit B12–> methylmalonyl CoA mutase
62
Q

How to cattle meet their needs for vits?

A
  • water-soluble vits are made by RMO

- Fat-soluble vits ADEK are obtained from diet or sun

63
Q

Ruminants are completely dependent on _____ to meet their glycogen and glucose need

A

Gluconeogenesis

64
Q

What is the % contribution of propinoate to glucose production?

A

70%

65
Q

What tissues require glucose in cattle?

A

Brain and RBC

66
Q

Ruminants’ predom fuel? (2)

A
  1. Acetate

2. Metabolism of VFA

67
Q

Monensin increases ___ and efficiency of feed util by 7.5%

A

Growth

68
Q

Explain how the following factors help contribute to the increase in grwoth and feed efficiency seen when using monensin:

  • Dec A:P ratio
  • Dec methan prod (30%)
  • Dec AA catabolism
A
  • Dec A:P ratio = MORE PROPIONATE FOR GLUCOSE PROD
  • Dec methan prod (30%) = 10% OF CALORIC VALUE IS LOST AS CH4
  • Dec AA catabolism = MORE AA AVAIL FOR PROTEIN SYN
69
Q

The acetyl coA used in FA synthesis comes from ___ and is generate in the cytosol using ATP citrate lyase

A

Glucose

70
Q

NADPH for FA synthesis is supplied by what 2 things? %?

A
  1. Malic enzyme (50%)

2. HMP shunt (50%)

71
Q

De novo FA syn occurs primarily in the ____ of ruminants

A

AT

72
Q

The acetyl coa used for FA synthesis comes from 2 of the VFA ___ and ___!! but NOT FROM PROPIONATE OR GLUCOGENIC AA

A

Acetate and Butyrate

73
Q

Ruminats lack the ATP=citrate lyase- unable to generate acetyl Coa in teh cytosol and foreced to use acetate made ______

A

In the rumen

74
Q

NADPH needed for FA biosyn suppliekd by what 2 things

A
  1. Cytoplasmic isocitrate dehydrogenase (ICDH)

2. HMP shunt

75
Q

The absense of ATP-citrate lyase and the presence of ICDH are signifcant evolutionary changes in ruminants relative to ___

A

Omnivores

76
Q

Rumen microorganisms? (4 functions)

A
  1. Use dietary lipids as sink for hydrogens
  2. Digest dietary protein to AA and ammonia
  3. Recycle urea
  4. Syn the EAA and water-soluble vits needed by ruminants
77
Q

Host ruminants does what 2 things?

A
  1. Uses RMO to supply their AA needs

2. Detoxifies ammonia generated in the rumen and the liver

78
Q

The primary function of dietary lipids is to ____? (3 things)

A
  1. Supply E
  2. EFF
  3. Fat- soluble vitamins
79
Q

Most of the ______ are digested (hydrolyzed) by lipolytic bacteria in the rumen

A

Dietary lipids

80
Q

FA made by host ruminant are similar to those made by other animals- specific to what 4 things?

A
  1. Even number C’s
  2. Linear (not branched)
  3. All dbl bonds are CIS with a KINK
  4. Methylene bridges (CH2) separate double bonds
81
Q

RMO form branched chain fatty acids (BCFA)- what are several characterstics of BCFA

A
  1. Made from methylmalonic acid
  2. Favor due to medium length BCFA
  3. Form some trasn dbl bonds with no kink
  4. Prod of conjug dbl bonds
82
Q

Because of the biohydrogenation phenomenon, ruminants have a high concentraton of _______ C16:0, C18:0, C18:1 - thus meat and milk from ruminants is high in saturate fat

A

Saturated fats

83
Q

Ruminal AA either ___ (40%) or used directly for bacterial protein synthesis (60%)

A

Fermented

84
Q

NPN in feedstuff are also convereted to ____ by RMO

A

Ammonia

85
Q

Generally, cattle can grow, reprod and lactate on a diety with only dietary NPN, however they would do better when given ________

A

Dietary protein

86
Q

Urea that is to be recycled reaches the rumen via what 2 things?

A
  1. Saliva

2. Diffusion across rumen wall

87
Q

Urea can only be used under what condition of the rumen?

A

The rumen has to have plenty of urease producing bacteria

88
Q

Urea has to be administered how often?

A

Daily to ensure that bacteria are present

89
Q

Urea levels are termed ______ aka (BUN)

A

Blood urea nitrogen

90
Q

BUN levels are a measure of ________________

A

Crude protein utilization

91
Q

If diet is low in crude protein, urea levels are ___

A

Low

92
Q

If diet is high in crude protein, the surplus AA are catabolized, increasing levels of ________ and ____ which has to be excreted

A

Ammonia and urea

93
Q

Which form is more readily absorbed? (NH4 or NH3)

A

NH3

94
Q

Fiber in the rumen has to be digested and fermented to do what?

A

Make room for next meal

95
Q

Insufficient lvls of RMO will do what things?

A

Slow down digestion and fermeantion and decrease rate of passage thru rumen

96
Q

What 2 things in stomach helps kill RMO?

A

Pepsin and acid

97
Q

How many genes coding lysozymes are in the cattle vs human?

A

Cattle has at least 10 genes (4 expressed in abomasum)

Humans have 1 gene

98
Q

What are “bypass proteins”?*

A

Dietary proteins specifically designed to escape fermentation in the rumen and to move on to SI for digestion and absorption

99
Q

___ is a natural bypass protein because suckling causes esophageal groove to close so milk goes directly to abomasum skipping the rumen

A

Milk

100
Q

Excess urea means excess ___ and this wastes energy as its coversion back to urea in the liver requires energy (___ATP/urea)

A

NH3, 4 ATP per urea

BIOCHEM FINALS** (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions