L2: biofilm in endodontics

Question 1

biofilm is formed from :

Answer 1

microcolonies
extrapolymeric substance (glycocolxmatrix)
water channels

Question 2

extrapolymeric substance % of pulp by volume ?

Answer 2

85%

Question 3

extraradicular biofilm causes:

Answer 3

purulent inflammation in peri radicular

sites:
teeth with asymptomatic periodontitis
periodontal abscess related to sinus tract

Question 4

periapical microbial biofilm:
where is it found and what are the microorganisms involved.

Answer 4

found in : periapical region of endodontically infected tooth , asymptomatic periodontal lesions

microorganisims involved:actinomyces , P. propionicum

Question 5

which material can get rid of biofilm by disturbing oxidative phosphorylation and inhibiting DNA synthesis of bacteria :

Answer 5

sodium hypocholrite

Question 6

cholohexidine :

Answer 6

effective against gram positive and gram negative bacteria due to its ability to form pores in membrane

-CANNOT destroy biofilm structure

-kills e faecalis in 30 seconds

Question 7

which material is effective at killing 1 day old e.faecalis but less effective in killing 3 week old biofilm ?

Answer 7

QMIX

Question 8

what are the uses of iodine and what is the normal concentrations used in endodontics?

Answer 8

2-5%
its a bactericidal , fungicidal, viricidal , and tuberculocidal and sporicidal agent . it functions by penetrating microorganism and attacking proteins ,nucleotides, and fatty acids, resulting in cell death

it is less effective against c.feacalis

Question 9

not obvious advantage of smear layer:

Answer 9

reduction of diffusion of fluids and prevent wetness of cut dentin surface