L2 - Autopsy & Labratory Diagnosis

Question 1

A ______ ________ is an examination of a dead body to determine the cause of death.

Answer 1

Post mortem

Question 2

Pathologist do a Post Mortem (PM) to:

A. Confirm/ reject clinical diagnosis
B. Collect tissues to conduct tests
C. Practice proper burial technique
D. Determine the cause of death
E. Identify what disease processes are occurring
E. A,B,D,E are correct
F. All of the above

Answer 2

E. A,B,D,E are correct

Pathologist do a Post Mortem (PM) to:
A. Confirm/ reject clinical diagnosis
B. Collect tissues to conduct tests
D. Determine the cause of death
E. Identify what disease processes are occurring

Question 3

When should you do a post mortem?

A. When other animals have also been effected
B. ASAP
C. Within a week
D. Never

Answer 3

B. ASAP

Question 4

Where should you perform a post mortem?

A. Areas difficult to disinfect
B. Areas which may contain food
C. High traffic areas
D. Dirt area in the sun

Answer 4

D. Dirt area in the sun

Note: Other areas include

• Concrete
• Straw bed (burn after)

Question 5

What should you do with the carcass after?

A. Transport to another area on the farm
B. Composting
C. Leave it
D. Put it in a nearby river

Answer 5

B. Composting

Question 6

What can you do to minimize exposures to pathogens during a PM?

A. Use high powered water then disinfect the area
B. Wear a hat
C. First disinfect the area then use high powered water to further clean the area

Answer 6

C. First disinfect the area then use high powered water to further clean the area

Question 7

All specimens should be handled and treated as though they are a potential zoonosis threat

True or False

Answer 7

True

Question 8

What biosafety level (BSL) is required for a routine necropsy of a domestic animal ?

A. 1
B. 2
C. 3
D. 4

Answer 8

B. 2

Question 9

Which BSL is required for an animal suspected to have Rabies?

A. 1
B. 2
C. 3
D. 4

Answer 9

C. 3

Question 10

For a person with immunosuppression/pregnancy BSL 3 is sufficient enough.

True or False

Answer 10

False

BSL 4 is used

Question 11

Which equipment is not needed to perform a necropsy?

A. Rib cutters
B. Saw or axe
C. Screw driver
D. Sharp knife

Answer 11

C. Screw driver

Question 12

Which PM steps for any species is wrong:

A. Perform external examination
B. Open the body cavities
C. Collect microbiologic samples
D. Remove and dissect organs
E. Collect histologic samples
F. Take pictures after not before

Answer 12

F. Take pictures after not before

Question 13

The EDTA (or purple top tube) is used for

A. Culture
B. Fluid analysis and cytology

Answer 13

B. Fluid analysis and cytology

Question 14

The Red top (or plain sterile tube) is used for

A. Culture
B. Fluid analysis and cytology

Answer 14

A. Culture

Question 15

Which regards to collecting histologic samples

A. A 10:1 ratio of formalin:tissue should be used
B. Tissue samples should be 1/2 cm or less
C. A 1:10 of formalin:tissue should be used
D. A,B are correct
E. B,C are correct

Answer 15

D. A,B are correct

A. A 10:1 ratio of formalin:tissue should be used
B. Tissue samples should be 1/2 cm or less

Question 16

Why shouldn’t a tissue sample be more than 1/2 cm ?

A. Because that’s standard protocol
B. Because any tissue bigger will not properly be seen histologically
C. Because formalin will not penetrate more than 1/2 cm
D. Because anything bigger won’t allow more tissue to fit in the jar

Answer 16

C. Because formalin will not penetrate more than 1/2 cm

Question 17

When packaging the samples don’t package histo separately instead freeze the formalin.

True or False?

Answer 17

False

Never freeze the formalin

Question 18

How do you confirm sepsis with culture ? (aka systemic bacterial infection in the body)

Answer 18

Tissues from all over the body should have the same bacteria infection if this is the case.

Question 19

When collecting micrbiologic samples (which is incorrect):

A. Specimen should be collect aseptically
B. Keep samples cold with ice/ gel packs
C. Collect samples from the intestine first
D. Place fresh tissue in separate containers

Answer 19

C. Collect samples from the intestine first

Note: Samples from the intestine should be collected last bc it will contaminate everything else if collected first.