L2 - Autopsy & Labratory Diagnosis Flashcards
A ______ ________ is an examination of a dead body to determine the cause of death.
Post mortem
Pathologist do a Post Mortem (PM) to:
A. Confirm/ reject clinical diagnosis B. Collect tissues to conduct tests C. Practice proper burial technique D. Determine the cause of death E. Identify what disease processes are occurring E. A,B,D,E are correct F. All of the above
E. A,B,D,E are correct
Pathologist do a Post Mortem (PM) to: A. Confirm/ reject clinical diagnosis B. Collect tissues to conduct tests D. Determine the cause of death E. Identify what disease processes are occurring
When should you do a post mortem?
A. When other animals have also been effected
B. ASAP
C. Within a week
D. Never
B. ASAP
Where should you perform a post mortem?
A. Areas difficult to disinfect
B. Areas which may contain food
C. High traffic areas
D. Dirt area in the sun
D. Dirt area in the sun
Note: Other areas include
- Concrete
- Straw bed (burn after)
What should you do with the carcass after?
A. Transport to another area on the farm
B. Composting
C. Leave it
D. Put it in a nearby river
B. Composting
What can you do to minimize exposures to pathogens during a PM?
A. Use high powered water then disinfect the area
B. Wear a hat
C. First disinfect the area then use high powered water to further clean the area
C. First disinfect the area then use high powered water to further clean the area
All specimens should be handled and treated as though they are a potential zoonosis threat
True or False
True
What biosafety level (BSL) is required for a routine necropsy of a domestic animal ?
A. 1
B. 2
C. 3
D. 4
B. 2
Which BSL is required for an animal suspected to have Rabies?
A. 1
B. 2
C. 3
D. 4
C. 3
For a person with immunosuppression/pregnancy BSL 3 is sufficient enough.
True or False
False
BSL 4 is used
Which equipment is not needed to perform a necropsy?
A. Rib cutters
B. Saw or axe
C. Screw driver
D. Sharp knife
C. Screw driver
Which PM steps for any species is wrong:
A. Perform external examination B. Open the body cavities C. Collect microbiologic samples D. Remove and dissect organs E. Collect histologic samples F. Take pictures after not before
F. Take pictures after not before
The EDTA (or purple top tube) is used for
A. Culture
B. Fluid analysis and cytology
B. Fluid analysis and cytology
The Red top (or plain sterile tube) is used for
A. Culture
B. Fluid analysis and cytology
A. Culture
Which regards to collecting histologic samples
A. A 10:1 ratio of formalin:tissue should be used
B. Tissue samples should be 1/2 cm or less
C. A 1:10 of formalin:tissue should be used
D. A,B are correct
E. B,C are correct
D. A,B are correct
A. A 10:1 ratio of formalin:tissue should be used
B. Tissue samples should be 1/2 cm or less
Why shouldn’t a tissue sample be more than 1/2 cm ?
A. Because that’s standard protocol
B. Because any tissue bigger will not properly be seen histologically
C. Because formalin will not penetrate more than 1/2 cm
D. Because anything bigger won’t allow more tissue to fit in the jar
C. Because formalin will not penetrate more than 1/2 cm
When packaging the samples don’t package histo separately instead freeze the formalin.
True or False?
False
Never freeze the formalin
How do you confirm sepsis with culture ? (aka systemic bacterial infection in the body)
Tissues from all over the body should have the same bacteria infection if this is the case.
When collecting micrbiologic samples (which is incorrect):
A. Specimen should be collect aseptically
B. Keep samples cold with ice/ gel packs
C. Collect samples from the intestine first
D. Place fresh tissue in separate containers
C. Collect samples from the intestine first
Note: Samples from the intestine should be collected last bc it will contaminate everything else if collected first.
