L18: Hematopoiesis Flashcards
Advantages for Immunfluorescent microscopy and flow cytometry?
IF microscopy
- shows localization of antigens and cell structure
- single marker
Flow cytometry
- quicker population count and sorting
- multiple markers can be sorted
Which cells are from a lymphoid lineage?
B/T cells, NK cells, plasma cells
Which cells are from a myeloid lineage?
RBC, Platelets, neutrophils, macropage, mast cells, eosino/baso-phils
which blood cells have the highest and lowest lifespan
RBC highest, neutrophils lowest
which blood cells have highest and lowest cell production rate?
RBC highest, lymphocytes lowets
Site of hematopoiesis in fetus?
0-2ms: yolk sac
2-7ms: liver and spleen
5-9ms: bone marrow
Site of hematopoiesis in infant?
All bone marrows. But red active cells gradually turn into inactive fat cells
Site of hematopoiesis in adult?
flat bones. But it can expand during the need of high cell production
what happens to the infant hematopoiesis sites as they grow?
red active cells gradually turn into inactive fat cells
Can adults ever increase their hematopoiesis site?
yes. during the need of high cell production
4 Definition of stem cells?
- proliferates
- self renewal
- differentiates into 2 or more cell types
- reconstitutes tissue damage
experiment to prove three of the stem cell properties in unknown cells
- lethal irradiation
- implant stem cells
- hemtapoesis in spleen
Are self renewing stem cells abundant?
No.
Do self renewing stem cells go through cell cycle often? When do they go through cell cycle if they ever do?
No they are mostly quiescent.
Which two protein expression is lowered in progenitor and differentiating cells?
CD34, Sca-1
