L11 - Electrophysiological Techniques

Question 1

Why record?

Answer 1

Extremely fast events

Extremely sensitive

Spatial resolution

Dissect details of individual

Question 2

Types of recording/complexity/where theyre suited for?

Answer 2

Extra: Easy/not specific - clinic

Intra: Difficult/specific - lab (neuron activity)

Path: V. difficult/v.specific - lab (channel activity)

Question 3

What kind of electrode is used?

Answer 3

Glass (<1um wide) pole heated

Tight seal = gigaown seal

Question 4

Who came up with this?

Answer 4

Neher and Sakmann

Question 5

What is cell-attached configuration?

Answer 5

Use: Record currents through limited (1-2) active channels

Question 6

What is inside-out configuration?

Answer 6

Use: Record current through single active channel away from cell (INTRACELLUALR FACE)

Rip off patch and stick it into a bath ( - agent) then add agent to modulate channel by acting on intra face e.g. kinase (+ agent)

Question 7

What is outside-out configuration?

Answer 7

Use: Record current through single active channel away from cell (EXTRACELLUALR FACE)

Rip off patch - hold it in the air (flips membrane) stick it in back - add agent that modulelates extra face e.g. NT

Question 8

Whole cell configuratinon

Answer 8

Use: Record currents through active channel in whole cell

Response to drugs